木蹄层孔菌免疫活性多糖超声提取工艺研究  

Process Optimization for Extraction of Immune Active Polysaccharides from Fomes fomentarius by Introduction of Ultrasonication

作　　者：何概易南 雷林生[1] 陈洁[1] 余传林[1] 任婧[1] 陈娜娜[1] 

机构地区：[1]南方医科大学药学院,广东广州510515

出　　处：《中药材》2011年第8期1277-1280,共4页Journal of Chinese Medicinal Materials

基　　金：广东省自然科学基金(07005189)

摘　　要：目的:研究超声处理在提取木蹄层孔菌多糖(简称:木蹄多糖)中的应用,并筛选出最佳提取工艺。方法:选用L9(34)正交表,对超声时间、水提温度及水提时间三因素各选三个水平进行组合,以多糖提取率、多糖含量及免疫刺激活性(刺激小鼠脾细胞代谢MTT活力)作为评价指标,找出最适提取条件。结果:超声处理对木蹄多糖免疫刺激活性的影响最为明显。以免疫刺激活性为指标,最佳提取工艺为:超声30 min,水提温度80℃,水提时间2 h。结论:超声处理可以作为木蹄免疫活性多糖提取的一种有效工艺。Objective：To study the optimal extraction process of immune active polysaccharides from Fomes fomentarius by introduction of ultrasonication.Methods：An orthogonal experimental design of L9（34） was used to investigate the effects of ultrasonication time,extraction temperature and extraction time on the extraction ratio,sugar content and immune stimulating activity（mouse splenocyte metabolic activity measured with MTT colorimetry） of the polysaccharides and the optimal extraction process was evaluated.Results： Ultrasonication treatment had the most remarkable effect on the immune stimulating activity of the polysaccharides.The optimal extraction process for extraction of immune active polysaccharides was as follows：ultrasonication for 30min,extraction temperature at 80℃ and water extraction time for 2h.Conclusion：Ultrasonication can be used as a useful technique for extraction of immune active polysaccharides from Fomes fomentarius.

关 键 词：木蹄层孔菌 多糖 超声提取 免疫活性 正交试验 

分 类 号：R284.2[医药卫生—中药学]