自体原位肝移植大鼠围术期急性肺损伤的研究  

Acute lung injury after orthotopic liver autotransplantation in SD rat model

作　　者：池信锦[1] 李玺[2] 葛缅[1] 张瑷兰[1] 罗刚健[1] 黑子清[1] 

机构地区：[1]中山大学附属第三医院麻醉科,广州510630 [2]中山大学附属第三医院甲乳外科,广州510630

出　　处：《中华实验外科杂志》2011年第11期1893-1895,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30972858、30801080）;广东省科技计划项目资助项目（2008B060600020、2009B050700021）;教育部博士点基金资助项目（200805580072）

摘　　要：目的建立SD大鼠自体原位肝移植模型，动态观察自体原位肝移植围术期肺损伤特点。方法选取健康SPF级SD大鼠40只，随机分为假手术对照组（S组，n=8）和模型组（M组），M组进行自体原位肝移植手术，根据肝脏再灌注时间不同再分为4个亚组。S组在麻醉后只进行开腹和血管的分离，不进行肝脏的阻断和灌注。分别在肝脏再灌注后4、8、16、24h采集动脉血和肺组织标本，分别用于检测动脉氧分压、肺组织病理学。结果与s组[（120．75±6．58）mmHg（1mmHg=0．133kPa）]比较，M2组（97．50±8．22）mmHg动脉氧分压降低（P〈0．05），其余各组差异无统计学意义（P〉0．05）。M组肺组织镜下见大量的炎症细胞浸润，肺泡腔内渗出明显，肺泡腔狭窄，肺间质明显出血充血，肺泡间隔明显增厚，肺实质所占的比例显著增加；这些病理改变在M2组（肝脏再灌注8h）达到高峰，然后逐渐好转。结论自体原位肝移植后SD大鼠存在肺损伤，在肝脏再灌注后8h达到高峰。Objective To investigate characteristics and regularity of lung injury after orthotopic liver autotransplantafion in the SD rat model. Methods Forty Sprague-Dawley rats were randomly divided into sham operated group（ Group S, n = 8 ） and model group（ Group M）. Aecording to the time of liver reperfusion, the rats in Group M were divided into 4 h after reperfusion （ Group M1, n = 8 ）, 8 h after reperfusion （ Group M2, n = 8）, 16 h after reperfusion （ Group M3, n = 8 ）, 24 h after reperfusion （ Group M4, n =8）. The rats in 4 model groups were anesthetized after repeffusion for 4 h, 8 h, 16 h, and 24 h respectively, and then collected the artery blood and lung tissue for the detection of PaO2 and morphologie changes of pulmonary pathology. Results Compared to Group S, there was a significant decrease of PaO2 in GroupM2 [（120.75 ±6.58） mmHg （1 mmHg=0.133 kPa） vs. （97.50±8.22） mmHg, P〈 0. 05 ], while there were no significant among Group S, M1, M3 and M4 （P 〉 0. 05 ）. Light mieroscope showed that plenty of inflammatory ceils infiltration, the obvious exudate in narrow alveolar space, the sig- nificant bleeding and hyperernia in pulmonary intersitital tissue, the thickening of alveolar interval, and much more lung parenchyma in the model groups, which was most signifieant in Group M2, and then improved gradually. Conclusion There existed lung injury after orthotopic liver autotransplantation for rats and showed most significanly at reperfusion for 8 h.

关 键 词：自体原位肝移植 急性肺损伤 病理学 

分 类 号：R657.3[医药卫生—外科学]