妊娠期间高叶酸饮食诱导子代小鼠先天性巨结肠模型的研究  

Mice models of congenital magacolon induced by high-dose folic acid diet at the duration of pregnancy

作　　者：吴川清[1] 林学科[1] 陶凯雄[1] 杜寒松[1] 韩高雄[1] 李航[1] 帅晓明[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院腔镜外科,武汉430022

出　　处：《中华实验外科杂志》2011年第11期1925-1926,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30872473）

摘　　要：目的观察母体妊娠期间高叶酸（FA）饮食在子代小鼠先天性巨结肠（HD）发生中的作用。方法分别用0（低叶酸组）、2（正常对照组），40、160、320mgFA／kg（高叶酸组）RHAA配方饲料补充孕小鼠，分别观察上述5组不同叶酸饮食环境下刚出生的仔鼠的结肠标本是否具有HD表型，并采取酶联免疫吸附试验（ELISA）检测孕鼠及仔鼠的血清叶酸浓度。结果随着饲料叶酸含量的升高，孕鼠及仔鼠血清叶酸浓度也呈相应逐渐升高，直至160mgFA／kg饲料饮食，母鼠及仔鼠体内叶酸水平达到最高值；低叶酸组与正常对照组仔鼠无巨结肠发生；在高叶酸组中：40、160、320mg3组巨结肠发生率分别为2．1％（1／42）、6．1％（2／33）、2．8％（1／36），并与仔鼠血清叶酸浓度密切相关。结论母体妊娠期间高叶酸饮食可以诱导仔鼠HD的发生，高叶酸饮食因素在HD发病机制中有着重要作用。Objective To evaluate the role of high-dose folic acid （FA） diet in the pathogenesis of congenital magacolon in a mouse model during pregnancy. Methods One group was supplemented with low-dose FA （0 mg FA/kg diet）, 3 groups were treated with high-dose FA supplentment （40, 160 and 320 mg FA/kg diet） , and the last goup was given normal dosage FA diet （2 mg FA/kg diet）. The blood serum FA concentration in pregnant female mice and filial generation mice were determined by using enzyme linked immunosorbent assay （ELISA）. Results As FA supplements increased, blood serum FA concentration of mother and filial generation mice was increased correspondingly. The 160 mg FA/kg diet-treated group had the highest blood serum concentration of mother and filial generation mice. Megacolon phenotype was only detected in high-FA dosage groups （40, 160 and 320 mg FA/kg diet）, with the positive incidence being 1/42 （2.4%）, 2/33 （6. 1% ） and 1/36 （2.8%） respectively. Conclusion High-dose FA can induce congenital magacolon of ,nice at the duration of pregnancy. High-dose FA diet may play an important role in the development of Hirchsprung＇ s disease.

关 键 词：叶酸 饮食 先天性巨结肠 

分 类 号：R714.2[医药卫生—妇产科学]