铁反应元件结合蛋白2基因在抗性及敏感品系淡色库蚊中表达量的分析  

Expression analysis of the iron responsive element binding protein 2 gene sequence in Culex pipiens pallens

作　　者：谭文彬[1] 王霄[2] 程鹏[3] 魏继波[3] 全芯[1] 

机构地区：[1]济宁医学院病原生物学教研室,山东济宁272010 [2]济宁医学院临床医学技能中心,山东济宁272010 [3]山东省寄生虫病防治研究所,山东济宁272033

出　　处：《中国现代医学杂志》2011年第18期2111-2113,共3页China Journal of Modern Medicine

基　　金：山东省教育厅课题(No:J09LF04);济宁市科委课题(2009年第18项)

摘　　要：目的进行不同品系的蚊中铁反应元件结合蛋白2(IREBP2)基因表达量的分析。方法根据二维电泳获得的肽段序列,设计引物扩增基因片段,荧光定量PCR检测在抗性及敏感品系蚊的表达水平差异。结果成功扩增淡色库蚊IREBP2基因的片段,荧光定量PCR表明IREBP2基因在抗性品系中的表达量是敏感品系的10.27倍。结论淡色库蚊的IREBP2基因可做为新的抗性检测及治理的基因靶标。[Objective] To analyze the iron responsive element binding protein 2 （IREBP2） gene expression level in different stains of mosquito. [Methods] Degenerate primers were designed on the basis of two-dimensional gel electrophoresis pepfide fragment sequence, fluorescence quantitative PCR was used to detect the expression levei difference between resistant and sensitive strains of mosquitoes. [Results] The 1REBP2 gene of Culex pipiens pallens were cloned successfully. FQ-PCR proved the IREBP2 gene expression level of resistant strairL is 10.27 times higher than that of sensitive strain. [ Conclusion ] Cuiex pipiens paliens IREBP2 gene can be used as a new gene target for resistance detection and treatment.

关 键 词：淡色库蚊 铁反应元件结合蛋白2 荧光定量PCR 

分 类 号：R115[医药卫生—公共卫生与预防医学]