二月桂酸二丁基锡对雄性大鼠肝细胞凋亡的作用  

Effect of Dibutyltin Dilaurate on Apoptosis of Liver Cells in Male Rats

作　　者：李兆辉[1] 倪亚杰[2] 王宏峰[1] 刘怀正[1] 栗学军[3] 

机构地区：[1]吉林省职业病防治院,吉林长春130021 [2]吉林省疾病预防控制中心 [3]吉林大学

出　　处：《中国卫生工程学》2011年第5期366-368,共3页Chinese Journal of Public Health Engineering

摘　　要：目的探讨二月桂酸二丁基锡(DBTD)对雄性大鼠肝细胞凋亡的作用。方法将雄性Wister大鼠随机分为对照组和3个不同剂量(5、10和20 mg/kg)的DBTD组。染毒组用色拉油配制成不同浓度的DBTD溶液,根据大鼠不同体重进行灌胃;对照组给予等量的色拉油;连续染毒8 w。在末次染毒结束后24 h(禁食12 h),股动脉取血后处死。以流式细胞术、细胞原位TUNEL法检测肝细胞凋亡率。结果染毒组大鼠随剂量的加大肝细胞DNA总损伤率明显提高(低:52.4%,中:81.4%,高:87.7%),与对照组(8.9%)比较,差异有统计学意义(P<0.05)。不同剂量组肝细胞各时相的细胞百分数发生变化:G0/G1期细胞百分数明显增加,G2/M期和S期细胞百分数明显减少,凋亡指数(AI)峰所占比例增加,染毒组与对照组比较差异有统计学意义(均P<0.05)。结论 DBTD可使雄性大鼠肝细胞DNA损伤率升高和DNA合成受抑制,使肝细胞出现G0/G1期阻滞,进入S期和G2/M期细胞数量减少,有丝分裂延迟,表明DBTD对大鼠肝细胞有凋亡作用。Objective To study the effect of Dibutyltin dilaurate（DBTD） on hepatocyte apoptosis in male rats.Methods The male rats were randomly divided into blank and test groups.Test groups of male rats were given different doses（5,10,20 mg/kg） of DBTD by gavage for 8 weeks respectively.Hepatocellular apoptosis was measured with flow cytometry.DNA damage was assessed by single cell gel electrophoresis（SCGE）.Results The different doses of DBTD could make the hepatocellular cycle change（low：52.4%,medium：81.4%,high：87.7%）,the difference between two groups was obvious（P0.05）.The cells in G0/G1 phases was observably increased.The cells in G2/M,S phases gradually taper in the hepatic cell of rats were increased with the increase of DBTD.The hepatocellular viscera coefficient was increased in the experimental groups to compare with that in the negative control.The results mentioned above were significant difference（P0.05）.Conclusion DBTD can inhibited hepatocellular DNA synthesis and causes the G1 block and cells mitotic delay,and make the cell enter S phase,the percentage of cells of G2 phase and M phase decreased with the increase of DBTD.It shows that DBTD may affect hepatocellular apoptosis of the rats.

关 键 词：DBTD 肝细胞 细胞凋亡 

分 类 号：R136[医药卫生—劳动卫生]