5-Ethynyl-2′-deoxyuridine as a molecular probe of cell proliferation for high-content siRNA screening assay by “click” chemistry  

作　　者：CHEN MiaoJuan QU DeZhong CHI WeiLin WANG Wei REN XiaoShuai CONG ShuJie LIANG PeiZhou FENG ShiPeng ZHANG BiLiang 

机构地区：[1]Lab of RNA Chemical Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences. Guangzhou 510530, China [2]State Key Laboratory of Respiratory Diseases Guangzhou Institute of Respiratory Diseases, Guangzhou 510182, China [3]Graduate University of Chinese Academy of Sciences, Beijing 100049, China [4]University of Science and Technology of China, School of Life Sciences, Hefei 230026, China [5]Guangzhou RiboBio Co., Ltd., Guangzhou Science Park, Guangzhou 510663, China

出　　处：《Science China Chemistry》2011年第11期1702-1710,共9页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China (30870535 and 90913017) (B. Zhang) ;Introduced Innovative R&D Team Program of Guangdong Province (Gene Silencing Technology and Therapeutics)

摘　　要：Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening （HCS） assays. Here we describe ethynyl deoxyuridine （EdU） as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.

关 键 词：molecular probe SIRNA miRNA ＂click＂ chemistry 

分 类 号：Q522[生物学—生物化学] TP212[自动化与计算机技术—检测技术与自动化装置]