Electrochemiluminescence glucose biosensor based on glucose dehydrogenase functionalized Ru(bpy)_3^(2+) doped silica nanoparticles  

作　　者：GUO GuangMei CHEN QingAi CHEN Xi 

机构地区：[1]College of Sciences, Hebei University of Science and Technology, Shijiazhuang 050018, China [2]Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China [3]Fujian Agriculture and Forestry University, College of Food Science, Fuzhou 350002, China

出　　处：《Science China Chemistry》2011年第11期1777-1781,共5页中国科学（化学英文版）

基　　金：financially supported by the Science and Technology Project of Hebei Province

摘　　要：A novel electrochemiluminescence (ECL) sensing approach was developed for glucose detection based on crosslinking Ru(bpy)3Cl2-doped silica nanoparticles (RuSiNPs) with glucose dehydrogenase on a glassy carbon electrode (GCE). Glutaral- dehyde and aminopropyltrimethoxysilane were used as linking agents, and chitosan was used to immobilize the composites onto the GCE surface. The ECL sensor presented good characteristics in terms of stability and reproducibility. Under opti- mized conditions, the linear response of ECL intensity to glucose concentration was valid in the range of 0.2 to 20 mmol/L (R2 = 0.9962). The application results indicated that the proposed approach is with great potential in the determination of glucose.A novel electrochemiluminescence （ECL） sensing approach was developed for glucose detection based on crosslinking Ru（bpy）3Cl2-doped silica nanoparticles （RuSiNPs） with glucose dehydrogenase on a glassy carbon electrode （GCE）. Glutaraldehyde and aminopropyltrimethoxysilane were used as linking agents, and chitosan was used to immobilize the composites onto the GCE surface. The ECL sensor presented good characteristics in terms of stability and reproducibility. Under opti- mized conditions, the linear response of ECL intensity to glucose concentration was valid in the range of 0.2 to 20 mmol/L （R2 = 0.9962）. The application results indicated that the proposed approach is with great potential in the determination of glucose.

关 键 词：ELECTROCHEMILUMINESCENCE sensing silica nanoparticles tris（2 2＇-bipridine） ruthenium GLUCOSE glucose dehydrogenase 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]