不同药物配方对公猪精液常温保存效果研究  

Effect of Storage in Ambient Temperature of Boar Semen Using Different Drug Formula

作　　者：陈玉霞[1] 孙克宁[1] 林峰[1] 杨婷[1] 高汉婷[1] 高腾云[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《家畜生态学报》2011年第4期65-67,共3页Journal of Domestic Animal Ecology

基　　金：河南省重点科技攻关计划项目(82102130005);河南省重大科技攻关计划项目(0422011200)

摘　　要：为提高猪精液的保存效果,为猪精液常温保存技术及稀释液配方的研究提供参考依据,设计了3种常温保存希释液配方,并对保存后的精子进行了顶体染色。结果表明:当精子活率为50%与30%时,配方Ⅱ的精子保存时间均显著高于配方Ⅰ与配方Ⅲ(P<0.05),且配方Ⅱ所保存精子的总存活时间也显著高于配方Ⅰ与配方Ⅲ(P<0.05)。上述结果表明,配方Ⅱ对猪精液的常温保存效果要优于配方Ⅰ与配方Ⅲ。In order to improve the storage effect of boar semen, three diluent formulas in ambient tem- perature was designed to preserve the boar semen, and the acrosome of the sperms was stained after they were preserved. The results showed that the storage time of the sperms stored by formulⅡ was obviously different（P〉0. 05） from that of formula I and formula Ⅲ when the sperm motility rate was 50%and 30% . The overall survival time of the sperms stored by formulaⅡ was also obviously different （P〈0.05） from that of formula I and formula Ⅲ.

关 键 词：公猪 精液 常温保存 稀释液 顶体染色 精子活率 

分 类 号：S811.5[农业科学—畜牧学]