三氧化二砷抑制小鼠B16黑色素瘤肺转移作用的研究  

Inhibitory Effect of Arsenic Trioxide on the Pulmonary Metastasis of Melanoma B16 Cells

作　　者：石莹[1] 夏俊[1] 杨同怀[1] 张玉心[1] 陈治文[1] 

机构地区：[1]安徽蚌埠医学院生化与分子生物学教研室,安徽233030

出　　处：《中国中西医结合杂志》2011年第10期1398-1400,1430,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：安徽省教育厅自然科学研究资助项目(No.2004kJ279);安徽省高校优秀青年人才基金项目(No.2009SQRZ129)

摘　　要：目的探讨三氧化二砷(As2O3)对小鼠B16黑色素瘤肺转移的抑制作用。方法 B16细胞静脉接种C57BL/6J小鼠,观察腹腔注射As2O3对小鼠肺组织重量及肿瘤肺转移结节的影响;HE染色、Ⅷ-RAg免疫组化染色观察肿瘤肺转移结节中微血管密度;CellTiter 96 Aqueous One试剂检测细胞黏附率。结果 As2O3能显著抑制小鼠B16黑色素瘤的肺转移。实验组和对照组肺组织重量分别为(0.139±0.013)g和(0.353±0.070)g;肺转移结节数分别为(20.42±1.78)个和(61.42±3.09)个;每个视野的微血管数分别为(3.25±0.75)个和(7.50±1.45)个;两组比较差异均有统计学意义(P<0.01)。As2O3对细胞黏附率有显著影响,两组比较差异有统计学意义(P<0.01)。结论 As2O3具有显著的抗肿瘤转移效应,可能与其抑制血管生成及增强细胞黏附有关。Objective To study the inhibitory effect of arsenic trioxide（As2O3） on the pulmonary metastasis of melanoma B16 cells.Methods Mice melanoma cells B16 were injected into the vein of the eye socket of C57BL/6J mice.The lung tissue weight and the B16 melanoma lung metastasis nodules were examined after intraperitoneal injection of As2O3.The microvessel density in the pulmonary metastatic tumor nodules was observed using HE staining and immunohistochemistry analysis for Ⅷ-R Ag.The cell adhesion rate was detected using CellTiter 96 Aqueous One reagent.Results As2O3 could significantly inhibit the pulmonary metastasis of B16 melanoma.The lung weight,the pulmonary metastasis nodules,and microvessels per visual field of the experimental group and the control group were 0.139±0.013 g and 0.353±0.070 g,20.42±1.78 and 61.42±3.09,3.25±0.75 and 7.50±1.45,respectively（all P0.01）.As2O3 showed significant effect on the cell adhesion rate,showing statistical difference between the two groups（P0.01）.Conclusions As2O3 had significant anti-tumor metastasis effect.It might be correlated with inhibiting angiogenesis and enhancing the cell adhesion.

关 键 词：三氧化二砷 B16黑色素瘤细胞 肿瘤转移 血管生成 细胞黏附 

分 类 号：R285[医药卫生—中药学]