露水草加工粒径和其β-蜕皮激素释放的关系  

Effect of Different Size of Ground Cyanotis arachnoidea Particles on the Release of β-ecdysone

作　　者：朱国栋[1] 张易祥[1] 叶金云[1] 吴成龙[1] 于岚[1] 杨霞[1] 

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000

出　　处：《安徽农业科学》2011年第29期17760-17761,共2页Journal of Anhui Agricultural Sciences

基　　金：浙江省重大国际合作项目(2009C14019);农业部"948"项目(2010-Z40);浙江省教育厅重大项目(ZD2009009);湖州市重大科技专项(2010ZD1002);国家自然科学基金(20977027);浙江省大学生科技创新计划(新苗人才计划)(2011R425009)

摘　　要：[目的]比较不同粒径露水草(Cyanotis arachnoidea C.B.Clarke)对所含β-蜕皮激素释放的影响。[方法]采用GRACE ODS C18色谱柱(250 mm×4.6 mm,5μm),流动相甲醇∶水=40∶60(V/V,%)洗脱。柱温26℃,流速1.0 ml/min,检测波长248 nm。[结果]相同来源的露水草粉体(粒径10、30和50μm)β-蜕皮激素含量检测值和普通细粉(粒径180μm)相比,粉体中蜕皮激素激素的检测值平均高1倍多。[结论]露水草粉体中的蜕皮激素更容易溶于甲醇,其提取率明显高于普通细粉。表明露水草经过超微粉碎后,细胞破壁率到得明显提高,更有利于有效成分的溶出。[Objective] The aim was to investigate the influence of different size of ground Cyanotis arachnoidea C.B.Clarke particles on the release of β-ecdysone.[Method] The content of β-ecdysone extracted from Cyanotis arachnoidea powders of different sizes was detected through HPLC,under the chromatographic conditions： GRACE ODS C18 column（250 mm × 4.6 mm,5 μm）,mobile phase methanol∶water = 40∶60（V/V,%）,column temperature 26 ℃,flow rate 1.0 ml/min and detection wavelength 248 nm.[Result] The extracted β-ecdysone from ultra-fine Cyanotis arachnoidea powder（particle size 10,30 and 50 μm） was as more than two fold as common powder（particle size 180 μm）.[Conclusion] The β-ecdysone in ultra-fine powder of Cyanotis arachnoidea was more easily soluble in methanol.The extraction rate of β-ecdysone was significantly higher than that of common powder.The result showed that the technology of ultra-fine pulverization markedly improved the dissolution of the β-ecdysone for increasing the cell breakage rate,and then the active ingredients could be extracted more easily.

关 键 词：高效液相色谱法 露水草(Cyanotis arachnoidea C.B.Clarke) β-蜕皮激素 

分 类 号：S132[农业科学—农业基础科学]