低温条件下一氧化氮参与了水杨酸对拟南芥生长的抑制作用  

Involvement of Nitric Oxide Production in the Inhibition Growth of by Salicylate at Chilling Temperature in Arabidopsis

作　　者：夏金婵[1,2] 何奕昆[2] 

机构地区：[1]河南中医学院,河南郑州450008 [2]首都师范大学,北京100048

出　　处：《安徽农业科学》2011年第29期17800-17801,17838,共3页Journal of Anhui Agricultural Sciences

基　　金：国家863计划(No.2002AA2Z1001);国家自然科学基金(Grant No.30328003);河南中医学院博士基金

摘　　要：[目的]验证NO是否参与了水杨酸对拟南芥生长的抑制过程,为植物在长期低温下生长的分子机制研究奠定基础。[方法]用可渗透细胞膜的NO特异性探针DAF-FM DA染色法测定4℃低温处理下,野生型拟南芥(Arabidopsis thaliana)和NahG突变体根中内源NO含量的变化。[结果]长期低温4℃处理可诱导植物中NO含量增加,并随时间的延长而升高;低温能够诱导野生型和NahG突变体中NO的产生,但体内水杨酸含量低的NahG突变体中NO含量明显低于比野生型。[结论]低温条件下NO参与了水杨酸对拟南芥生长的抑制过程。[Objective] The aim was to investigate whether NO is involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis.[Method] The endogenous NO level in roots of wild-type Arabidopsis thaliana and NahG mutant growing at 4 ℃ was measured with NO-specific probe DAF-FM DA staining method.[Result] NO level in plants growing at 4 ℃ for a long term could be increased with time;low temperature could induce NO production in wild-type and NahG mutants of A.thaliana,in which the NO level in NahG mutant with low level of salicylic acid was significantly lower than that in wild-type.[Conclusion] NO was involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis at low temperature.

关 键 词：拟南芥 低温 水杨酸 一氧化氮 

分 类 号：S132[农业科学—农业基础科学]