分光光度法测定角果藜中总黄酮含量  

Determination of Total Flavones Content in Ceratocarpus arenarius L.by Spectrophotometry

作　　者：刘珊珊[1] 马先梅[1] 杨红兵[1] 

机构地区：[1]石河子大学化学化工学院新疆兵团化工绿色过程重点实验室,新疆石河子832003

出　　处：《安徽农业科学》2011年第29期17865-17866,共2页Journal of Anhui Agricultural Sciences

基　　金：新疆兵团国际科技合作计划(2011BC003)

摘　　要：[目的]建立角果藜中总黄酮含量的测定方法。[方法]采用加热回流法提取角果藜中的黄酮类化合物,以芦丁为标准品测定总黄酮含量。[结果]采用紫外分光光度法测定总黄酮含量时,在0.019～0.102 mg/ml浓度范围内线性关系良好(r=0.999 1)。方法的精密度和准确度均较高,相对标准偏差为0.226%,平均回收率为99.36%。测得角果藜中总黄酮含量为10.12 mg/g。[结论]该方法操作简单,科学准确,适合用于角果藜中总黄酮含量的测定。[Objective] The aim was to establish the method for determining total flavoens content in Ceratocarpus arenarius L..[Method ] Flavonoids were extracted from C.arenarius by Heating refluxing,and determined with rutin as the standard.[Result] In the range of 0.019-0.102 mg/ml,linear relationship was good（r=0.999 1）.This method had higher precision and accuracy with RSD of 0.226% and recoveries of 99.36%.The total flavoens content from C.arenarius is 10.12 mg/g.[Conclusion] The method was simple and reliable,which could be used to control the quality of C.arenariu.

关 键 词：角果藜 黄酮类化合物 分光光度法 

分 类 号：O657.32[理学—分析化学]