杂交鳢(乌鳢♂×斑鳢♀)及其亲本对2种病原菌的抗感染能力比较  

Comparison on Anti-infection of Hybrid Snakehead(Channa argus ♂× Channa maculate ♀) and Its Parents to Two Pathogenic Bacteria

作　　者：林启存[1] 冯晓宇[1] 蔡丽娟[1] 许宝青[1] 刘凯[1] 

机构地区：[1]杭州市农业科学研究院,浙江杭州310024

出　　处：《安徽农业科学》2011年第29期17942-17943,17948,共3页Journal of Anhui Agricultural Sciences

基　　金：杭州市重大科技创新项目"杂交鳢优势利用及配套养殖技术研究与示范"(20083212A04)

摘　　要：[目的]分析评价杂交鳢及亲本对病原菌的抗病力水平。[方法]通过人工感染试验方法,开展了杂交鳢(乌鳢♂×斑鳢♀)及其亲本对两种病原菌的抗感染试验。[结果]以嗜水气单胞菌为试验菌株、菌液浓度9×108 cfu/ml(100、10-1、10-2、10-3 4个稀释度)、注射剂量0.5 ml时,3 d内杂交鳢LD50平均值介于乌鳢与斑鳢间,且差异均不显著;以诺卡氏菌为试验菌株、菌液浓度7×109 cfu/ml(100、10-1、10-2、10-3 4个稀释度)、注射剂量0.5 ml时,30 d内杂交鳢LD50平均值介于斑鳢与乌鳢间,且差异也均不显著。[结论]高浓度感染时,嗜水气单胞菌、诺卡氏菌对3种鳢鱼均有极强的致病性,108 cfu/ml菌液浓度时,3种鳢鱼死亡率达100%;不过低浓度感染时致死率明显减弱,且不同品种对病原菌的耐受力有些差异。[Objective ] The study aimed to analyze and evaluation the anti-infective level of to the pathogenic bacteria.[Method] The test of anti-infection to 2 pathogenic bacteria was conducted on the hybrid snakehead and its parents through the artificial infection test method.[Results]When Aeromonas hydrophila was the tested strain,the bacterium fluid was 9×108 cfu/ml in which there were 4 dilution grade of 100,10-1,10-2 and 10-3,the injection dosage was 0.5 ml/fish,the median lethal dose（LD50） on the hybrid snakehead was between C.argus and C.maculate,and no significant difference was found among the strains.When Nocardia seriolea was the tested strain,the bacterium fluid was 7×109 cfu/ml in which there were 4 dilution grade of 100,10-1,10-2 and 10-3,the injection dosage was 0.5 ml/fish,the median lethal dose（LD50） on hybrid snakehead was between C.maculate and C.argus,and no significant difference was found among the strains.[Conclusion] When the bacterium fluid was at high levels of infection,both A.hydrophila and Nocardia seriolea were highly pathogenic to 3 kinds of the snakehead fish.When the bacterium fluid was 108 cfu/ml,the mortality rate of the snakehead fish was 100%.When the fluid was at low level of infection,the mortality rate was significantly weakened.The different varieties had some different tolerance to the pathogenic bacteria.

关 键 词：杂交鳢 嗜水气单胞菌 诺卡氏菌 抗感染能力 

分 类 号：S941[农业科学—水产养殖]