一株水华鱼腥藻溶藻菌的分离鉴定及菌藻关系初探  

Identification of an Algae-lysing Bacterium of Anabaena flosaquae and Primary Research on Their Relationship

作　　者：叶姜瑜[1,2] 钟以蓉[1] 俞岚[3] 李文娟[1] 

机构地区：[1]重庆大学城市建设与环境工程学院,重庆400045 [2]三峡库区生态环境教育部重点实验室,重庆400045 [3]金华职业技术学院,浙江金华321017

出　　处：《安徽农业科学》2011年第29期18121-18124,共4页Journal of Anhui Agricultural Sciences

基　　金：三峡工程生态环境建设与保护试点示范专项计划

摘　　要：[目的]研究溶藻特性及菌藻关系,为进一步研究溶藻细菌对水华的治理作用提供帮助。[方法]从富营养化水体中分离得到一株有高效溶藻效果的菌株(S7),研究了其对水华鱼腥藻(Anabaena flosaquae)的抑制效果、作用方式和不同环境因子对溶藻效果的影响,以及菌藻关系,并对菌株进行了菌体Poly-p染色、革兰氏染色和分子鉴定。[结果]菌株投加量为藻液量的30%时,7 d叶绿素a的去除率达到90%以上。pH为9、温度35℃下藻的去除率最高。S7菌株与水华鱼腥藻形成竞争共栖的生态关系,并通过分泌溶藻物质间接抑制水华鱼腥藻生长,且该物质具有一定的热稳定性。根据生理生化及16S rDNA序列分析鉴定,S7属于金黄杆菌属(Chryseobacteriumsp.)。[结论]该菌对水华鱼腥藻有较强的溶藻效果,且为聚磷菌。[Objective] The aim was to explore the relationship between alage-lysing bacterium and Anabaena flosaquae so as to provide reference for the control of bloom.[Method] An algae-lysing bacterium strain named S7 was isolated from eutrophic river.The lytic efficiency and performing mode of S7 strain to Anabaena flosaquae was studied.Influence of different environmental factors and the relationship between S7 strain and Anabaena flosaquae was also studied,and then the bacterium strain was physiologically identified.[Result] More than 90% of Anabaena flosaquae had been removed by 7 d when the volume ratio of medium to algae solution was 30%,the pH was 9 and the temperature was 35 ℃.These results also showed that a mutual inhibit relationship existed between S7 strain and Anabaena flosaquae.The S7 strain killed the algae by indirectly through certain lytic agents in absence of direct contact with the target but by secreting metabolites.Moreover,these lytic agents also had the thermostability.16S rDNA sequence analysis showed that S7 strain belonged to Chryseobacterium sp..[Conclusion] The examine Poly-p proved that S7 strain was polyphosphate accumulating bacteria（PAOs）and produced better lytic efficiency.

关 键 词：溶藻细菌 水华鱼腥藻 溶藻特性 菌藻关系 金黄杆菌 

分 类 号：S132[农业科学—农业基础科学]