一种基于混流控制的多细胞三维受控组装系统  

Design of a Three-dimensionally Controlled Multi-cell-assembly System Based on the Control of a Mixer Nozzle

作　　者：王秋君[1] 徐铭恩[1] 李艳蕾[1] 袁梅娟[1] 胡金夫[1] 

机构地区：[1]杭州电子科技大学生物医学工程与仪器研究所,杭州310018

出　　处：《生物医学工程学杂志》2011年第5期1030-1034,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30600140;30970738);浙江省科技计划项目资助(2008C23007);浙江省自然科学基金资助项目(Y2091074)

摘　　要：细胞三维受控组装技术为体外构建活体组织器官提供了理论依据和技术支持。功能性组织和器官是多种细胞的有序三维排列,具有复杂的空间结构,因而在体外构建具有生物活性的组织器官,就需要细胞三维受控组装技术能够在空间上实现多种细胞材料的精确定位组装。本研究面向多细胞组装的需求,在单喷头细胞组装系统基础上,设计了新的混流喷头系统以及脉冲切换控制电路,开发出多细胞三维受控组装系统,并基于该系统进行了多组分的类细胞外基质材料的堆积实验。研究结果证明该系统可以准确地进行多细胞三维受控组装,达到构建非均质结构体的目的。Three-dimensionally controlled cell-assembly technique makes fabricating tissues and organs in vitro to be possible. However, for real tissues and organs with complex structure and various cells, fabricating tissues and organs in vitro need a technique that could assemble and locate multi cells and materials precisely in the space. Facing the needs of multi-cell assembly, we designed a mixer nozzle and the matching pulse switching circuit which based on the single-nozzle cell assembly system, and developed a multi-cell-assembly system. We also carried out some assembly experiments with this system using materials that were similar to the multi-component extracellular matrix materials. The results demonstrated that the system could assemble various cells and materials into three-dimensional inhomogeneous structures precisely.

关 键 词：多细胞组装 混流喷头 脉冲切换 

分 类 号：R318.1[医药卫生—生物医学工程]