水稻土中铁还原菌多样性  

Phylogenetic diversity of dissimilatory Fe(III)-reducing bacteria in paddy soil

作　　者：黎慧娟[1,2] 彭静静[1,2] 

机构地区：[1]中国科学院城市环境研究所城市环境与健康重点实验室,福建厦门361021 [2]中国科学院研究生院,北京100039

出　　处：《应用生态学报》2011年第10期2705-2710,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金重点项目(41090282)资助

摘　　要：微生物介导的异化Fe(III)还原是非硫厌氧环境中Fe(III)还原生成Fe(II)的主要途径,然而相关的铁还原菌还不是很清楚,特别是在水稻土中.本文采用富集培养的方法,以乙酸和氢气作为电子供体,水铁矿和针铁矿作为电子受体,通过末端限制性片段长度多态性(T-RFLP)技术和16SrRNA基因克隆测序相结合的分子生物学方法研究了水稻土中铁还原菌的多样性.结果表明:无论是以乙酸或氢气为电子供体,水铁矿或针铁矿为电子受体,地杆菌(Geobacter)和梭菌(Clostridiales)是富集到的主要微生物群落;乙酸为电子供体时,富集到的主要微生物群落还包括红环菌(Rhodocyclaceae);因此,除地杆菌外,梭菌和红环菌很可能也是水稻土中重要的铁还原菌.Microorganism-mediated dissimilatory Fe（III） reduction is recognized as the dominant mechanism for Fe（III） reduction to Fe（II） in non-sulfidogenic anaerobic environments, but the microorganisms involved, especially in paddy soil, are still poorly understood. In this paper, an enrichment culture was conducted to study the phylogenetic diversity of Fe（III）-reducing bacteria in paddy soil, with acetate or hydrogen as the electron donor and with ferrihydrite or goethite as the electron acceptor, and by the methods of terminal-restriction fragment length polymorphism （T-RFLP） technology and 16S rRNA genes cloning and sequencing. No matter what the electron donor and electron acceptor were supplemented, the most abundant microorganisms were Geobacter and Clostridiales, and Rhodocyclaceae were also abundant, when acetate was supplemented as electron donor, which suggested that besides Geobacter, Clostridiales and Rhodocyclaceae could be also the important Fe（III）-reducing bacteria in paddy soil.

关 键 词：水稻土 异化铁还原菌 富集培养 末端限制性片段长度多态性(T-RFLP)技术 

分 类 号：S154.3[农业科学—土壤学]