β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸支架复合骨髓基质细胞修复节段性骨缺损  

Repair of long segment bone defects with a novel bone tissue engineering scaffold beta-tricalcium phosphate/calcium polyphosphate fiber/poly-L-lactic acid composite and bone marrow mesenchymal stem cells

作　　者：李毅[1] 梁晓军[1] 王坤正[2] 陈君长[2] 杨团民[1] 同志超[1] 

机构地区：[1]西安市红十字会医院,陕西省西安市710054 [2]西安交通大学附属第二医院骨科,陕西省西安市710004

出　　处：《中国组织工程研究与临床康复》2011年第38期7036-7040,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:组织工程β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸支架材料具有良好的生物相容性。目的:评估骨髓基质细胞与β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸复合体修复兔桡骨大段骨缺损成骨的效果。方法:取新西兰大白兔40只,建立桡骨双侧大段骨缺损模型,其中35只右侧植入自体骨髓基质细胞与β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸复合物作为实验组,左侧植入β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸支架材料作为对照组;另5只作为空白对照不作任何处理。植入后4,8,12,16周拍摄X射线片观察骨缺损修复情况。结果与结论:实验组术后2周可见缺损处有散在的、少量模糊状骨痂生成,术后4周可见明显骨生成影像,成云雾状,均匀分布在骨缺损区,术后8周整个缺损区均可见骨痂生成,成骨现象更加明显,部分髓腔已通,术后12～16周,缺损区已完全被新生骨组织充填,骨髓腔已完全再通,修复区较正常桡骨细,骨缺损修复效果明显优于对照组与空白对照组(P<0.01)。说明自体骨髓基质细胞与β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸复合移植可较完全修复大节段骨缺损。BACKGROUND：Bone tissue engineering scaffold beta-tricalcium phosphate/calcium polyphosphate fiber/poly-L-lactic acid（β-TCP/CPPF/PLLA） composite has good biocompatibility.OBJECTIVE：To explore the effect of β-TCP/CPPF/PLLA scaffold and bone marrow mesenchymal stem cells（BMSCs） in repairing rabbit long segment bone defects.METHODS：Forty New Zealand white rabbits were divided into BMSCs/β-TCP/CPPF/PLLA group（group A）,pure β-TCP/CPPF/PLLA group（group B） and contrast group（group C） randomly.Animal models of long segment radius bone defect were established.The BMSCs/β-TCP/CPPF/PLLA were implanted into the right side of the radius defect（group A）,while the pure β-TCP/CPPF/PLLA were implanted into the left side（group B） as control.We did nothing with the contrast group.The rabbits were sacrificed respectively after 4,8,12,16 weeks and the X-ray film was performed at the same time to evaluate the repair effect in different groups.RESULTS AND CONCLUSION：X-ray film showed there was a small quantity of clouding bony callus in defect region after 2 weeks in group A.Marked osteogenesis screenage could be seen after 4 weeks.The defect region was filled with neonate osseous tissue completely and the bone medullary cavity was completely recanalized during 12-16 weeks.The defect region was filled partly in group B and group C,while group B was significant than group C（P 0.01）.X-ray score revealed that group A was much higher than group B at any time（P 0.01）.The BMSCs/β-TCP/CPPF/PLLA composite were capable of repairing long segment bone defects.

关 键 词：骨髓基质细胞 支架材料 组织工程 自体移植 骨缺损 修复 

分 类 号：R318[医药卫生—生物医学工程]