幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性  

Preparation and in vitro release characteristics of chitosan microspheres loading Helicobacter pylori whole cell protein antigen

作　　者：刘微[1,2] 谢勇[2] 刘志军 

机构地区：[1]新余市人民医院消化内科,江西省新余市338025 [2]南昌大学第一附属医院消化疾病研究所江西省消化疾病研究重点实验室,江西省南昌市330006

出　　处：《中国组织工程研究与临床康复》2011年第38期7085-7089,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助课题(30460052)~~

摘　　要：背景:不同方法制备出的幽门螺杆菌全菌蛋白抗原壳聚糖微球,其包裹率和控释效果也不同。目的:探讨幽门螺杆菌全菌蛋白抗原壳聚糖微球的最佳制备方案,观察其体外释放特性。方法:使用Berthold沉淀法制备壳聚糖微球,筛选最佳制备方案;使用扫描电镜及粒径分析仪观察壳聚糖微球的形态及粒径分布;冻干后的壳聚糖微球包裹幽门螺杆菌全菌蛋白抗原,使用BCA蛋白定量试剂盒测量分析微球的抗原包裹率、包裹量及释放率。结果与结论:从32种壳聚糖微球制备方案中筛选出了以海得贝壳聚糖为原料、冰乙酸的浓度为1%、硫酸钠为沉淀剂、pH值为5.0、不进行超声处理方案为最佳制备方案,扫描电镜示微球光滑圆整、致密,粒径分布在1.0～5.0μm;抗原包裹率为79.92%,包裹量为16.47%;体外释放实验表明,总抗原释放率为20.39%,呈缓慢释放状态。BACKGROUND：Chitosan microspheres loading Helicobacter pylori whole cell protein antigen prepared by different methods have different the package rates and controlled-release effects.OBJECTIVE：To prepare chitosan microspheres loading Helicobacter pylori whole cell protein antigen and observe the release characteristics in vitro.METHODS：Berthold precipitation method was used to prepare chitosan microspheres and the preparation was optimized;scanning electron microscope and particle size analyzer were used to observe the shape and size measurement of chitosan microspheres;the BCA Protein Assay Kit was used to measure the loading efficiency,loading capacity and releasing rate of microspheres.RESULTS AND CONCLUSION：We chose the best preparation program of chitosan microspheres from 32 species preparation programs：the best preparation program was the material of sea shell chitosan,1% concentration of acetic acid,sodium sulfate for precipitating agent and 5.0 pH value,non-use of ultrasonic treatment.SEM results showed that the microspheres presented with smooth round and dense,diameters ranged from 1.0 μm to 5.0 μm;Antigen loading efficiency was 79.92%,loading capacity was 16.47%;Release experiment in vitro showed that the total antigen releasing rate was 20.39%,showing a slow-release status.

关 键 词：壳聚糖 幽门螺杆菌 微球 包裹 释放 

分 类 号：R318[医药卫生—生物医学工程]