富含半胱氨酸的酸性分泌糖蛋白对瘢痕疙瘩成纤维细胞β1转化生长因子和I型胶原蛋白基因表达的影响  

Effects of SPARC on expression of TGF-β1 and collagen type I in keloid fibroblasts

作　　者：王春[1] 李小静[1] 刘超华[1] 翁晓娟[1] 郝冬月[1] 

机构地区：[1]安徽医科大学第一附属医院整形美容外科,合肥230032

出　　处：《中华医学美学美容杂志》2011年第5期377-380,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：基金项目：安徽省自然科学基金资助项目（编号：070413102）

摘　　要：目的探讨实时荧光定量逆转录-聚合酶链反应（RT—PCR）法检测富含半胱氨酸的酸性分泌糖蛋白（secreted protein，acidic and rich in cysteine，SPARC）对瘢痕疙瘩成纤维细胞β1转化生长因子（TGF-β1）、I型胶原蛋白基因表达的影响。方法用不同浓度重组人SPARC作用于体外培养的瘢痕疙瘩成纤维细胞，并以空白组为对照，实时荧光定量RT-PCR法检测TGF-βI、I型胶原蛋白mRNA的表达。结果与空白对照组相比，实验组TGF-βI、I型胶原蛋白mRNA表达明显增高（P〈0．05）。结论SPARC能显著促进瘢痕疙瘩成纤维细胞TGF-β1、I型胶原蛋白基因的表达。Objective To observe the effects of secreted protein, acidic and rich in cysteine （SPARC） on the expression of TGF-β1 and collagen type I in cultured human keloid fibroblasts by real-time fluorescence quantitative RT-PCR. Methods In vitro keloid fibroblasts were stimulated by different concentrations of recombinant human SPARC, and with the control group for comparison, real-time fluorescence quantitative RT-PCR to detect expression of TGF-β1 and collagen type I . Results Compared with the control group, the expression of TGF-β1 and collagen type I was significantly increased in the experimental group. Conclusions SPARC could enhance the expression of TGF-β1 and collagen type I in keloid fibroblasts significantly.

关 键 词：瘢痕疙瘩 富含半胱氨酸的酸性分泌糖蛋白 实时荧光定量逆转录-聚合酶链反应 Β1转化生长因子 I型胶原蛋白 

分 类 号：R622[医药卫生—整形外科]