血卟啉单甲醚-光动力疗法诱导瘢痕成纤维细胞凋亡  

Apoptosis of hypertrophic scar fibroblasts induced by HMME-PDT

作　　者：蔡宏[1] 顾瑛[2] 曾晶[2] 赵广[1] 

机构地区：[1]解放军空军总医院皮肤科,北京100142 [2]解放军总医院激光医学科

出　　处：《中华医学美学美容杂志》2011年第5期381-384,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的探讨血卟啉单甲醚-光动力疗法（HMME—PDT）诱导瘢痕成纤维细胞（HSF）的凋亡效应。方法将手术切除的瘢痕组织进行原代培养，建立增生性HSF的传代细胞系，取第4～6代HSF爬片后分为HMME—PDT组（实验组）和对照组，Hoechst 33258染色后在荧光显微镜下观察HMME-PDT后HSF形态学变化；Annexin V—FITC和碘化丙啶（PI）双染后经流式细胞仪（FCM）检测HMME-PDF后HSF凋亡率和坏死率。结果HMMEPDT处理后核染色质高度凝聚，呈团块颗粒状，或呈波纹状、折缝样改变；Annexin V-FITC和PI双染结果表明，PDT后凋亡率显著升高[（34．82±1．42）％VN（3.12±0．28）%，P〈0．05]，并伴有细胞坏死的发生，但组内坏死率显著低于凋亡率[（14．65±1．02）％VS（34．82±1．42）％，P〈0．05]。结论经HMME-PDT（治疗参数：HMME：4ug／ml．照射波长630nm，功率密度10mW／cm2，能量密度2．5J／cm2）处理后HSF发生死亡，其中凋亡率显著高于坏死率，HMME-PDT能诱导原代培养HSF凋亡。Objective To investigate the apoptotic effects of hypertrophic scar fibroblast （HSF） induced by HMME-PDT. Methods Fibroblasts were cuhured from nontreated hypertrophic scars. and cells at passages 4-6 were used for the experiments （photosensitizer dose 4 ug/ml, ）,630 nm, pow er density 10 mw/cm2, energy fluence 2.5 J/cm2）. Morphological and biochemical changes in fibroblasts were assessed by Hoechst 33258 staining and fluorescence microscopy. The rate of apoptotic or necrotic cells was detected by flow cytometry （FCM） through double staining of Annexin V FITC and popodium iodide （PI）, respectively. Results Marked morphological features of cell apoptosis were viewed under the fluorescent microscope through Hoeehst 33258 staining. The analysis of FCM indica ted that the apoptotic rate was significantly increased after HMME-PDT [（ 34.82±1.42） % vs （3.12±0.28） %, P〈0.05], and apoptotic rate was higher than necrosis rate [（14. 65±1.02） % vs （34.82± 1. ,12） %, P〈0.05]. Conclusions Low-level exposure to 630 nm PDT mediated by HMME appears to induce fibrohlast apoptosis.

关 键 词：血卟啉单甲醚 瘢痕成纤维细胞 光动力疗法 凋亡 

分 类 号：R318.08[医药卫生—生物医学工程]