STAg与BCG-DNA和氢氧化铝佐剂联合免疫小鼠的免疫效果  

Immune Effect of STAg Combined with BCG-DNA and Aluminum Hydroxide in Mice

作　　者：张影[1] 杨英超[1] 张瑾[1] 薄淑英[1] 辛晓芳[1] 王国治[1] 

机构地区：[1]中国食品药品检定研究院细菌一室,北京100050

出　　处：《中国生物制品学杂志》2011年第10期1177-1179,共3页Chinese Journal of Biologicals

摘　　要：目的观察弓形虫可溶性速殖子抗原(Soluble tachyzoite antigen,STAg)与BCG-DNA和A(lOH)3佐剂联合免疫小鼠的免疫效果。方法将BALB/c小鼠随机分为5组,分别经小鼠头背部皮下注射20μg STAg、50μg BCG-DNA+20μgSTAg、50μg A(lOH)3+20μg STAg、50μg BCG-DNA+50μg A(lOH)3+20μg STAg,对照组注射生理盐水,共免疫4次。末次免疫后1周处死小鼠,采血分离血清,并制备脾细胞悬液,ELISA法测定小鼠血清IgG值及抗体效价;ELISPOT法检测分泌特异性IFNγ的淋巴细胞数量。结果 A(lOH)3+BCG-DNA+STAg组小鼠血清抗体效价和IgG值及产生特异性IFNγ的淋巴细胞数量均显著高于STAg组、BCG-DNA+STAg组和A(lOH)3+STAg组(P<0.05)。结论 STAg联合BCG-DNA和A(lOH)3佐剂能诱导小鼠产生更强的体液免疫和细胞免疫应答,A(lOH)3与BCG-DNA佐剂对弓形虫STAg具有免疫协同效应。Objective To observe the immune effect of soluble tachyzoite antigen（STAg） of Toxoplasma gondii combined with BCG-DNA and aluminum hydroxide in mice.Methods BALB / c mice were divided into five groups randomly.The mice in four test groups were injected s.c.with 20 μg STAg,50 μg BCG-DNA ＋ 20 μg STAg,50 μg Al（OH）3 ＋ 20 μg STAg and 50 μg BCG-DNA ＋ 50 μg Al（OH）3 ＋ 20 μg STAg,respectively,while those in control group with physiological saline,for 4 times.The mice were killed one week after the last injection,of which the sera were separated,and the splenotye suspensions were prepared.The level and titer of serum IgG was determined by ELISA,while the count of lymphocytes secreting specific IFNγ by ELISPOT method.Results The titer and level of serum IgG as well as the count of lymphocytes secreting specific IFNγ of mice in Al（OH）3 ＋ BCG-DNA ＋ STAg group were significantly higher than those in STAg,BCG-DNA ＋ STAg and Al（OH）3 ＋ STAg groups（P 0.05）.Conclusion STAg combined with BCG-DNA and aluminum hydroxide induced strong humoral and cellular immune responses in mice as compared with STAg alone,and aluminum hydroxide and BCG-DNA adjuvant showed synergistic action with STAg.

关 键 词：刚地弓形虫 可溶性速殖子抗原 BCG-DNA 氢氧化铝 佐剂 免疫 

分 类 号：R382.5[医药卫生—医学寄生虫学] R392-33[医药卫生—基础医学]