血浆蛋白质组学研究中样品前处理技术的研究进展  

Progress in Technique for Pretreatment of Test Samples in Research on Plasma Proteomics

作　　者：肖忠华[1] 邱宗荫[2] 

机构地区：[1]重庆三峡医药高等专科学校,重庆404120 [2]重庆医科大学药学院,重庆400016

出　　处：《中国生物制品学杂志》2011年第10期1243-1248,共6页Chinese Journal of Biologicals

摘　　要：由于血浆/血清蛋白来源广泛,种类和数量繁多,丰度差异大,动态范围宽,给血浆/血清蛋白全谱蛋白的分离、检测和运用差异蛋白组学筛选肿瘤的生物标志物带来了困难,而血浆/血清蛋白前处理成为血浆蛋白质组学研究面临的首要问题。近年来,研究者对血浆蛋白质组学研究中分析对象(血浆或血清)的选择、收集、处理、存储,高丰度蛋白的去除,低丰度蛋白的富集和蛋白分级等前处理技术方面取得了很大的进展,本文就上述领域的研究进展作一综述。Sample pretreatment is the principal challenging to human plasma proteomics research,which brings difficulties to the separation and determination of full spectrum of plasma or serum proteins as well as screening of biomarker of tumors by differential proteomic,since human plasma or serum contain elements of all proteins produced in the body characterised the wide dynamic range and obviously differential in abundance.In this article,we present an overview of the effects of the variables involved in sample collection,handling and storage and different approaches for removal of high abundance proteins and serum / plasma fractionation.

关 键 词：血浆 蛋白质组学 样品前处理 高丰度蛋白 低丰度蛋白 

分 类 号：R392-33[医药卫生—免疫学]