鸡核受体辅激活蛋白基因(NCOA1)SNPs分析及mRNA的表达  

SNPs Analysis and mRNA Expression Level of Nuclear receptor Co- activator 1 Gene (NCOA1) in Chicken

作　　者：黎寿丰[1] 赵振华[1] 黄华云[1] 李春苗[1] 张静[1] 薛龙岗[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225003

出　　处：《农业生物技术学报》2011年第5期832-836,共5页Journal of Agricultural Biotechnology

基　　金：江苏省"333"工程培养资金项目(No.BRA2010162);现代农业产业技术体系建设专项资金;中国农业科学院家禽所博士基金(No.JQ2009002)共同资助

摘　　要：核受体辅激活蛋白基因(NCOA1)是类固醇激素受体超家族中的一员,在生殖细胞的生长和分化过程中起着重要的调节作用。本研究利用PCR-SSCP技术对NCOA1基因外显子的多态性进行了分析,在第3和12外显子上分别发现了SNPs位点,并采用实时荧光定量PCR对多态性位点处mRNA表达水平进行了研究,发现NCOA1基因随着生长发育其表达量呈波动性增加,14周龄前表达量很少,随着性成熟NCOA1基因表达量快速增加,28周龄时其表达量达到峰值,之后表达量下降。产蛋性能较好的母系鸡NCOA1基因的相对表达量比产蛋性能较差的父系鸡较早地开始增加,并且在14周龄后表达量显著高于父系鸡(P<0.05)。同位点对产蛋性能有显著影响的优势基因型AA和CC个体的表达量在16周后也显著高于同位点的其他基因型个体(P<0.05)。研究提示NCOA1基因mRNA表达水平影响鸡的性成熟过程和产蛋性能。Nuclear receptor co-activator 1 gene （NCOA1） is one of the members of steroid hormone receptor which is essential for growth and differentiation of ovarian follicles. Single nucleotide polymorphisms （SNPs） were detected by PCR-SSCP, and two SNPs was found at exon 3 and 12. Rea1-time quantitative PCR was used for identification of expression changes of NCOA1 gene at exon 3 and 12. Results indicated that NCOA1 gene expression was increased along with growth as undulatory property and few before 14 weeks. NCOA1 gene expression was rapidly increased along with sex maturation, and high expression was found at 28 weeks then decreased. NCOA1 expression level of female line was easier than that of male line and significantly higher mRNA expression after 14 weeks （P0.05）. The advantage genotyps of AA and CC which significantly influenced the egg performance of individual expression, was significantly higher than that of the other genotype within the same site after 16 weeks （P0.05）. NCOA1 genes may play an important role in the process of sexual maturation and egg production performance.

关 键 词：鸡 核受体辅激活蛋白基因(NCOA1) PCR-SSCP 实时荧光定量PCR 

分 类 号：S78[农业科学—林学]