淫羊藿对激素性股骨头坏死骨组织OPG/RANKL mRNA表达的影响  

Effect of epimedium extract on osteoprotegerin and RANKL mRNA expressions in glucocorticoid-induced femoral head necrosis in rats

作　　者：王建忠[1] 高鸿雁[1] 王坤正[2] 周荣兴[1] 李晓东[1] 郭军[1] 吕慧成[1] 

机构地区：[1]内蒙古医学院第二附属医院创伤骨科,内蒙古呼和浩特010030 [2]1西安交通大学医学院第二附属医院骨外科,陕西西安710004

出　　处：《南方医科大学学报》2011年第10期1714-1717,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81160228)~~

摘　　要：目的观察糖皮质激素对大鼠股骨头骨组织中骨保护素(OPG)/核因子kappa B受体活化因子配基(RANKL)mRNA表达的影响及淫羊藿的拮抗作用,探讨淫羊藿预防激素性股骨头坏死的作用效果及其作用机制。方法健康SD大鼠48只,雌雄各半,随机分为激素组、淫羊藿组和对照组,每组16只。激素组和淫羊藿组每只给予肌肉注射醋酸泼尼松龙12.5mg/kg,2次/周,淫羊藿组同时给予淫羊藿提取液1 ml/100 g(相当于0.1 g/ml生药)灌胃,而激素组用生理盐水代替淫羊藿灌胃,1次/d;对照组用生理盐水代替激素和淫羊藿以同样方式肌注和灌胃。4周后取左侧股骨头骨组织石蜡包埋,HE染色,鉴定股骨头坏死情况;取右侧股骨头骨组织提取总RNA,采用实时定量聚合酶链反应技术检测OPG和RANKLmRNA表达水平,计算出OPG/RANKL比值。结果激素组、淫羊藿组、对照组大鼠出现股骨头坏死表现比例分别为71.43%(10/14)、26.67%(4/15)、0(0/16);激素组、淫羊藿组、对照组OPG mRNA表达分别为1.13±0.32、1.47±0.42和1.51±0.43;RANKL mRNA表达分别为2.70±1.24、1.82±0.46和1.55±0.42。激素组OPG和OPG/RANKL比值低于淫羊藿和对照组,而RANKL表达高于淫羊藿组和对照组,差异有统计学意义(P<0.05)。淫羊藿组与对照组比较,差异无统计学意义。结论淫羊藿预防激素性股骨头坏死可能与其拮抗皮质激素导致的OPG/RANKL mRNA表达异常有关。Objective To investigate the effect of glucocorticoid on the expression levels of osteoprotegerin（OPG） and receptor activator of nuclear factor kappa B ligand（RANKL） mRNAs in rat femoral head and the antagonistic effect of Epimedium,and explore the mechanism of Epimedium in preventing glucocorticoid-induced femoral head necrosis.Methods Forty-eight adult SD rats were randomized into glucocorticoid group,Epimedium group and control group.In the former two groups,the rats received intramuscular injection of 12.5 mg prednisolone twice a week,and in Epimedium group,additional 1 ml/100 g aqueous Epimedium extract（equivalent to 0.1 g/ml of the crude drug） was administered intragastrically once daily.The control group received only intramuscular saline injection.After 4 weeks of treatment,osteonecrosis of the left femoral head was detected by HE staining,and the right femoral head was sampled for detection of OPG and RANKL mRNA expressions using real-time quantitative PCR.Results In glucocorticoid,Epimedium and control groups,the mortality rate of the rats was 12.5%（2/16）,6.25%（1/16）,0（0/16）,and femoral head necrosis occurred at a rate of 71.43%（10/14）,26.67%（4/15）,and 0（0/16）,respectively.In glucocorticoid group,the expression level of OPG mRNA was significantly lower,RANKL expression significantly higher,and OPG/RANKL ratio significantly lower than those in Epimedium and control groups（P0.05）.OPG,RANKL and their ratios showed no significant differences between Epimedium group and the control group.Conlusion Epimedium can prevent glucocorticoid-induced femoral head necrosis probably by antagonizing glucocorticiod-induced abnormal expressions of OPG and RANKL mRNA.

关 键 词：淫羊藿 糖皮质激素 股骨头 骨坏死 骨保护素 核因子kappaB受体活化因子配基 

分 类 号：R285.5[医药卫生—中药学]