醋酸铅诱导人肝细胞系L-02细胞凋亡与caspase-3表达关系的影响  

Lead acetate induces cell apoptosis and promotes caspase-3 expression in human liver cell line L-02

作　　者：邢伟[1] 李胜联[2] 陈兆夷[1] 

机构地区：[1]桂林医学院附属医院消化内科,广西壮族自治区桂林市541004 [2]桂林医学院公共卫生学院,广西壮族自治区桂林市541004

出　　处：《世界华人消化杂志》2011年第23期2426-2431,共6页World Chinese Journal of Digestology

基　　金：广西自然科学基金资助项目;No.桂科自0640184~~

摘　　要：目的:探讨醋酸铅对人肝细胞系L-02细胞凋亡与caspase-3表达的关系.方法:不同浓度(0、2.5、40、100、200、400μmol/L)的醋酸铅处理的L-02细胞24h及(或)48h后,MTT法观察L-02细胞生长的影响,Hoechest33258染色法观察细胞凋亡形态学变化,RT-PCR和Westernblot法观察caspase-3的表达.结果:24h及48h后与对照组比较,醋酸铅能明显抑制细胞的增殖(24h:0.4678±0.0438,0.4686±0.0733,0.4500±0.0712,0.4244±0.0407,0.3998±0.0499vs0.5234±0.0589;48h:0.4290±0.0607,0.4184±0.0296,0.4032±0.0499,0.3856±0.0386,0.3750±0.0149vs0.5300±0.0397,P<0.05或0.01).48h后各实验组Hoechest33258染色,细胞呈凋亡形态学改变;与对照组比较,caspase-3mRNA的表达明显升高,呈明显的剂量-效应关系(1.0912±0.0769,1.2874±0.144,1.4536±0.1046,1.6986±0.1371,1.9882±0.0925vs0.8438±0.0933,P<0.01),caspase-3蛋白质水平表达升高.结论:醋酸铅能有效抑制L-02细胞的增殖,并诱导其凋亡,其机制可能与caspase-3活化有关.AIM： To investigate the impact of lead acetate on cell apoptosis and caspase-3 expression in human liver cell line L-02. METHODS： After L-02 cells were treated with different concentrations （0, 2.5, 40, 100, 200, 400 μmol/L） of lead acetate for 24 or 48 h, the prolif- eration of L-02 cells was assayed by MTT assay; cell morphological changes were observed after Hoechest33258 staining; and the expression of caspase-3 mRNA and protein was determined by RT-PCR and Western blot. RESULTS： Compared to the normal control group, the proliferation of L-02 cells was significantly inhibited after treatment with different concentrations of lead acetate for 24 or 48 h （24 h： 0.4678 ± 0.0438, 0.4686 ± 0.0733, 0.4500 ± 0.0712, 0.4244 ± 0.0407, 0.3998 ± 0.0499 vs 0.5234 ± 0.0589; 48 h： 0.4290 ± 0.0607, 0.4184 ± 0.0296, 0.4032 ± 0.0499, 0.3856 ± 0.0386, 0.3750 ± 0.0149 vs 0.5300 ± 0.0397, all P 〈 0.05 or 0.01）. Significant morphological changes were noted in cells treated with lead acetate for 48 h. Compared to the normal control group, the levels of caspase-3 mRNA increased significantly in a dose-dependent manner in L-02 cells treated with lead acetate （1.0912 ± 0.0769, 1.2874 ± 0.144, 1.4536 ± 0.1046, 1.6986 ± 0.1371, 1.9882 ± 0.0925 vs 0.8438 ± 0.0933, all P 〈 0.01）. Additionally, the expression levels of caspase-3 protein also significantly increased after treatment with lead acetate. CONCLUSION： Lead acetate inhibits proliferation and induces apoptosis of L-02 cells possibly via a mechanism associated with the activation of caspase-3.

关 键 词：醋酸铅 L-02细胞 细胞凋亡 天冬氨酸特异性半胱氨酸蛋白酶-3 

分 类 号：R114[医药卫生—卫生毒理学]