地塞米松对TNF-α诱导的H460细胞增殖的影响  

Effect of dexamethasone on TNF-α stimulated generation of H460 cell

作　　者：朱运奎[1] 杨娜[1] 李继东[1] 金远林[1] 刘杜姣[1] 李超然[2] 

机构地区：[1]兰州军区兰州总医院呼吸内科,甘肃兰州730050 [2]兰州大学第二临床医学院

出　　处：《西北国防医学杂志》2011年第5期321-323,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：甘肃省自然科学基金资助项目(SZS051-A25-088)

摘　　要：目的:观察地塞米松对TNF-α诱导的肺癌H460细胞增殖的影响。方法:体外培养H460细胞,经TNF-α刺激后,随机分为对照组和实验组,对照组用不含地塞米松的DMEM培养液培养,实验组则分别加入含不同浓度地塞米松的DMEM培养液。通过MTT比色实验观察各组的值。结果:TNF-α(12.5～100 ng/ml)可促进H460增殖(P<0.01),而地塞米松(25～200μg/ml)可以抑制这种作用(P<0.01)。结论:对于TNF-α诱导肺癌H460细胞增殖而言地塞米松是一种良好的抑制剂。Objective：To investigate the effect of dexamethasone （DEX） on TNF-a stimulated generation of H460 cell. Methods： The H460 （ Human non - small lung cancer cell） was cultured and stimulated by TNF - a in vitro, and was randomly divided into control and experimental group. The experimental group was cultured in DMEM media with different concentrations of dexamethasone. The inhibitions of DEX on the generation of H460 cell stimulated by TNF-or were examined by tetrazolium salt （MTY） assay. Results： TNF -a（ 12.5 to 100 ng/ ml）can enhance H460 generation with a concentration - dependent manner（P 〈0.01 ）. DEX （25 to 200 μg/ml） can inhibit this generation of H460 cultured with TNF - a （P 〈 0.01 ）. Conclusion： DEX may be a good inhibitor for the generation of H460 cell stimulated by TNF - a.

关 键 词：肺肿瘤 H460细胞 细胞增殖 肿瘤坏死因子-Α 地塞米松 

分 类 号：R96[医药卫生—药理学]