胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白骨修复材料的性质评估  

Properties evaluation of collagen-hydroxyapatite-chondroitin sulfate-bone morphogenetic protein bone substitute material

作　　者：王浩[1] 张里程[1] 石涛[1] 熊琦[1] 唐佩福[1] 

机构地区：[1]中国人民解放军总医院骨科,北京100853

出　　处：《北京大学学报（医学版）》2011年第5期730-734,共5页Journal of Peking University:Health Sciences

摘　　要：目的:评估以胶原、羟基磷灰石、硫酸软骨素等3种天然骨骼基本成分构建成的三维多孔骨修复材料的理化性能和体内生物学性能,观察其作为骨形态发生蛋白(bone morphogenetic protein 2,BMP-2)载体的效果。方法:以胶原、羟基磷灰石、硫酸软骨素及BMP-2为原料,通过化学交联和冷冻干燥的方法构建具有一定三维结构的骨修复材料。通过HE染色、扫描电镜观察材料的结构性能;通过表面能谱、X线衍射观察材料的理化性能;将骨髓基质干细胞(marrow stromal cells,MSCs)种植在材料表面,观察MSCs在材料表面的粘附、增生和分化;将该复合材料种植在大鼠体内,观察材料在体内的降解和异位成骨情况。结果:骨修复材料在植入局部保持完整的支架结构,具有利于细胞粘附和增殖的多孔结构。通过肌肉埋植,在异位诱导形成了骨组织,并且随着骨组织的形成,支架逐渐降解吸收。结论:胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白是具有良好的生物相容性和骨诱导特性的骨修复材料。Objective：To construct a new 3D porous bone substitute material with collagen,hydroxy-apatite and chondroitin sulfate,which has the main components of nature bone and the cell growth factor BMP-2 with bone inductive ability.Methods： Collagen-hydroxyapatite-chondroitin sulfate scaffolds were prepared by chemical cross linking and freeze-drying,and bone morphogenetic protein（BMP） was incorporated into the scaffolds by adsorption.The bone substitute material was investigated by HE analysis,scanning electron microscope（SEM）,electron spectroscopy for chemical analysis（ESCA）,and X-ray diffraction（XRD）.Rat mesenchymal stem cells（MSCs） were seeded into the scaffolds and cultured to form cell/scaffold（CS） constructs in vitro.The ectopic osteoinduction of the scaffolds were evaluated in vivo.Results： The bone substitute material had a porous 3D structure facilitating cells growing into it.Implanted into the muscle,the scaffolds were degraded with the forming of new bone.Conclusion： Our Findings indicate that the bone substitute material has good biocompatibility and its attachment to CS could improve the adhesion and differentiation of cells.

关 键 词：骨代用品 胶原 羟基磷灰石 软骨素硫酸盐类 骨形态发生蛋白质类 

分 类 号：R318.17[医药卫生—生物医学工程]