三氯乙烯对肝细胞代谢酶基因与凋亡基因表达的影响  

mRNA expression of hepatic metabolic enzyme genes and apoptosis genes in L02 cells treated with trichloroethylene

作　　者：徐新云[1] 刘月峰[1] 柯跃斌[1] 毛吉炎[1] 袁建辉[1] 周丽[1] 杨淋清[1] 杨细飞[1] 黄海燕[1] 洪文旭[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518055

出　　处：《癌变．畸变．突变》2011年第5期349-352,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：深圳市科技计划项目(200802073)

摘　　要：目的:探讨三氯乙烯(trichloroethylene,TCE)对人L02肝细胞代谢酶基因CYP1A2、CYP3A4、CYP2E1和凋亡基因BAX、BAD、Bcl-2 mRNA表达水平的影响。方法:以1%二甲亚砜(DMSO)为溶剂对照,用不同浓度TCE(0.25、0.5、1.0和2.0mmol/L)染毒L02肝细胞24h,另外用同一浓度1.0mmol/L TCE染毒细胞3～24h,采用实时荧光定量PCR(real-time quantitative PCR)技术检测L02肝细胞中CYP1A2、CYP3A4、CYP2E1和凋亡基因BAX、BAD、Bcl-2 mRNA的表达。结果:不同剂量TCE染毒后CYP1A2、CYP3A4、CYP2E1 mRNA表达明显上升,CYP1A2升高36%～726%,CYP3A4升高56%～525%,CYP2E1升高15%～94%,与对照组比较,差异均有统计学意义(P<0.05或P<0.01);凋亡基因BAX、BAD、Bcl-2表达水平也明显升高,与对照组比较差异均有统计学意义(P<0.05或P<0.01)。用1.0mmol/L TCE染毒3～24h,同样发现肝代谢酶基因和凋亡基因表达增加,与对照组比较差异均有统计学意义(P<0.05或P<0.01)。结论:TCE可诱导L02肝细胞中代谢酶基因及凋亡基因表达变化,肝代谢酶基因和凋亡基因可能在TCE对肝细胞毒性效应中发挥重要作用。OBJECTIVE：To study mRNA expression of hepatic metabolic enzyme genes and apoptosis genes in L02 cells after treatment with trichloroethylene（TCE）.METHODS：Liver cells（L02 cells） were cultured with various doses of TCE（0.25,0.5,1.0 and 2.0 mmol/L） for 24 h,or with a single dose of TCE（1.0 mmol/L） for different periods of time（3 h,6 h,12 h,24 h）,the control was treated with DMSO.Real-time fluorescent PCR assay was applied for detection of mRNA expression of hepatic metabolic enzyme genes（CYP1A2,CYP3A4,CYP2E1） and apoptosis genes （BAX,BAD,Bcl-2）.RESULTS：The relative levels of mRNA expression of hepatic metabolic enzyme genes and apoptosis genes markedly increased after TCE treatment at various doses for 24 h.The elevation of mRNA expression in TCE treatment groups was significant in comparison with control（P0.05,or P0.01）.Additionally,when the cells were treated with a single dose of TCE（1.0 mmol/L） for different periods of time,the mRNA expression also increased significantly compared with control（P0.05,or P0.01）.CONCLUSION：Trichloroethylene could induce changes in mRNA expression of hepatic metabolic enzyme genes and apoptosis genes.These genes might play important roles in cytotoxicity of trichloroethylene.

关 键 词：三氯乙烯 L-02肝细胞 代谢酶 凋亡基因 

分 类 号：Q786[生物学—分子生物学] R994.6[医药卫生—毒理学]