亲和素-生物素黏附系统修饰纳米纤维构建组织工程神经的实验研究  

An experimental study of tissue engineered nerve using ABBS modified nanofibers

作　　者：刘俊[1] 阎作勤[1] 

机构地区：[1]复旦大学附属中山医院骨科,上海200032

出　　处：《中华手外科杂志》2011年第5期300-302,共3页Chinese Journal of Hand Surgery

摘　　要：目的观察亲和素．生物素黏附系统（avidin-blotinbinding system，ABBS）快速黏附雪旺细胞（SCs）对组织工程神经促进神经再生的影响。方法以坐骨神经缺损10innl作为实验模型。取40只Wistar大鼠分成A、B、C、D4组，每组10只。A组：自体神经移植修复组；B组：空白对照组，用聚乳酸己内酮共聚物[Poly（L-lacticacid-co-epsilon-caprolactone），P（LLA-CL）]支架修复神经缺损；C组：P（LLA-CL）支架上用普通方法黏附雪旺细胞修复神经缺损；D组：P（LLA-L）支架上用ABBS法黏附雪旺细胞修复神经缺损。术后3个月，检测坐骨神经功能指数、神经传导速度、再生神经轴突及髓鞘厚度，评估ABBS对于促进神经再生的影响。结果c组与D组的神经修复效果无差别。虽然修复效果不及A组，但是均明显好于没有雪旺细胞的B组。结论ABBS在动物体内实验中未对组织工程神经促进神经再生造成不利影响，是一种可靠的细胞快速黏附方法。Objective To observe the effect of Schwann cells attached by avidin-biolin binding system （ABBS） on regeneration of tissue engineered nerve. Methods Sciatic nerve 10 mm defect model was created in 40 Wister rats that were divided into four groups. In group A the defect was repaired by autologous nerve graft. In group B the defect was repaired with Poly （ L-lactic acid-co-epsilon-caprolactone） [ P （LLA-CL） ] scaffold. In group C the defect was brideged with P（LLA-CL） scaffold filled with Schwann cells. In group D the defect was bridged by P（LLA-CL） scaffold attached with Schwann cells by avidin-biotin. The sciatic functional index （SFI）, nerve conduction velocity, the number of nerve fibers and thickness of myelin sheath were measured 3 months postoperatively. Results There was no difference between groups C and D. The results of both groups were better than those of the no Schwann cell group B, but worse than those of the autograft group A. Conclusion ABBS does not have adverse effects on nerve regeneration using tissue-engineered nerve in the in vivo application and is a reliable method to attach cells to biomatefials.

关 键 词：生物素 神经再生 亲和素 聚乳酸己内酮共聚物 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]