昼夜节律基因hPer1和hPer2对食管癌细胞株Eca-109放射敏感性的影响  

Effects of Circadian Rhythm Genes hPer1 and hPer2 on Radiosensibility of Eca-109 Cells

作　　者：孙凤飞[1] 朱家媛[2] 刘康[1] 李婷婷[2] 李德智[1] 刘延友[3] 李光明[1] 

机构地区：[1]川北医学院第二临床医院,四川南充637000 [2]川北医学院,四川南充637000 [3]四川大学华西医学中心基础医学与法医学院,四川成都610041

出　　处：《航天医学与医学工程》2011年第5期332-335,共4页Space Medicine & Medical Engineering

基　　金：四川省南充市科学技术计划项目资助(2010SF10)

摘　　要：目的探讨昼夜节律基因hPer1和hPer2对食管癌细胞株(Eca-109)放射敏感性的影响。方法体外培养食管癌Eca-109细胞株,通过避光和乙酰肉豆蔻佛波酯(PMA)分别诱导昼夜节律基因hPer1和hPer2节律性的表达,用RT-PCR检测出表达高峰和低谷。在hPer1和hPer2表达的高峰和低谷时,分别用6mV-X射线给予照射,通过Tunel及流式细胞术,检测肿瘤细胞的凋亡。结果昼夜节律基因表达高峰时照射与低谷时照射比较,肿瘤细胞凋亡率减少(P<0.05)。结论昼夜节律基因hPer1和hPer2高表达,能够降低食管癌细胞的放射敏感性。Objective To investigate the effects of circadian rhythm genes-hPer1 and hPer2 on radiosensibility of esophageal cancer cell lines Eca-109.Methods The circadian rhythm expression of hPer1 of esophageal cancer cell lines,Eca-109,was induced with dark-culture.The circadian rhythm expression of hPer2 of Eca-109 was induced with phorbol 12-myristate 13-acetate（PMA）.The expression of hPer1 and hPer2 was detected with PT-PCR.Eca-109 cells were irradiated at peak（PG-hPer1,PG-hPer2） and nadir（NG-hPer1,NG-hPer2） of hPer1 and hPer2 with 6mV-X-ray respectively.The apoptosis rate of Eca-109 were evaluated with TUNEL and flow cytometry.Results The apoptosis rate of Eca-109 cells of PG-hPer1 and PG-hPer2 were lower than those of NG-hPer1 and NG-hPer2（P0.05）.Conclusion hPer1 and hPer2 over-expression can decrease the radiosensibility of esophageal cancer cell lines Eca-109.

关 键 词：昼夜节律基因 食管癌 ECA-109细胞 凋亡 放射敏感性 

分 类 号：R852.6[医药卫生—航空、航天与航海医学]