受翻译调节的肿瘤蛋白在PRL-3促进结肠癌转移中的作用  

Role of translationally controlled tumor protein in PRL-3-promoted metastasis of colon cancer cells

作　　者：褚忠华[1] 刘璐[1] 来伟[1] 李守峰[1] 曾育杰[1] 关玉峰 

机构地区：[1]中山大学孙逸仙纪念医院胃肠外科,广东广州510120 [2]广州市番禺中心医院普通外科,广东广州511400

出　　处：《中国病理生理杂志》2011年第10期1907-1912,共6页Chinese Journal of Pathophysiology

基　　金：广东省社会发展计划基金资助项目(No.2007B031513012);广东省自然科学基金资助项目(No.9151008901000058);广东省自然科学基金资助项目(No.10151008901000071)

摘　　要：目的:研究受翻译调节的肿瘤蛋白(TCTP)在肝再生磷酸酶-3(PRL-3)促进结肠癌细胞增殖、迁移和侵袭中的作用。方法:构建载体pAcGFP-C3-PRL-3及pAcGFP-C3并分别转染至结肠癌LoVo细胞中,获得稳定表达PRL-3的LoVo-PRL-3细胞及对照细胞LoVo-control。Western blotting及real-time PCR检测2种细胞PRL-3及TCTP表达。设计并合成特异性干扰TCTP mRNA的siRNA序列(TCTP-siRNA)和阴性对照序列(control-siRNA),并将siRNA瞬时转染至LoVo-PRL-3细胞。在转染siRNA后24 h、48 h和72 h,Western blot-ting及real-time PCR检测LoVo-PRL-3细胞TCTP表达。CCK8-8和Transwell方法检测LoVo-control、LoVo-PRL-3及转染TCTP-siRNA和转染control-siRNA的LoVo-PRL-3细胞间增殖、迁移和侵袭能力差异。结果:转染PRL-3可以显著上调LoVo细胞TCTP mRNA和蛋白的表达(P<0.05)。TCTP-siRNA在转染后的24 h、48h和72 h可以有效抑制LoVo-PRL-3细胞TCTP mRNA表达(P<0.01),同样TCTP蛋白在转染后的48 h和72 h也显著受到抑制(P<0.01)。转染PRL-3能显著促进LoVo细胞增殖、迁移和侵袭能力(P<0.05),然而siRNA干扰抑制上调的TCTP表达后又能显著抑制LoVo-PRL-3细胞增殖、迁移和侵袭能力(P<0.05)。结论:PRL-3通过上调TCTP表达促进结肠癌细胞增殖、迁移和侵袭。用siRNA靶向抑制TCTP表达可能是预防和治疗结肠癌转移的有效手段。AIM：To study the role of translationally controlled tumor protein（TCTP） in the proliferation,migration and invasion of phosphatase of regenerating liver-3（PRL-3）-promoted colon cancer cells.METHODS： The vectors pAcGFP-C3 and pAcGFP-C3-PRL-3 were constructed and transfected into the colon cancer cell line LoVo.LoVo-PRL-3 cells stably expressing PRL-3 and LoVo-control cells were established.The expression levels of PRL-3 and TCTP in both cells were detected by Western blotting and real-time PCR.The specific siRNA sequence for TCTP mRNA and control-siRNA were synthesized and transfected into the LoVo-PRL-3 cells.TCTP expression at mRNA and protein levels in LoVo-PRL-3 was detected by Western blotting and real-time PCR 24 h,48 h and 72 h after transfection.The proliferation,migration and invasion abilities of LoVo-control cells,LoVo-PRL-3 cells,TCTP-siRNA and control-siRNA cells were detected by CCK-8 assay and the method of Transwell cell culture chambers.RESULTS： The expression of TCTP at mRNA and protein levels in LoVo cells was significantly increased after PRL-3 transfection（P〈0.05）.TCTP mRNA was significantly inhibited 24 h,48 h and 72 h after transfection of TCTP-siRNA（P〈0.01）.TCTP protein was also significantly inhibited 48 h and 72 h after transfection（P〈0.01）.Compared with LoVo-control cells,the proliferation,migration and invasion abilities of LoVo-PRL-3 cells were significantly enhanced（P〈0.05）.However,lowering the up-regulated expression of TCTP in LoVo-PRL-3 cells inhibited the proliferation,migration and invasion abilities（P〈0.05）.CONCLUSION： PRL-3 promotes proliferation,migration and invasion of colon cancer cells by up-regulating the TCTP expression.siRNA targeting TCTP may be an effective method for prevention and treatment of colon cancer cell metastasis.

关 键 词：肝再生磷酸酶-3 受翻译调节的肿瘤蛋白质 结肠肿瘤 肿瘤转移 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]