NGF预处理对AMI大鼠心肌VEGF及其受体KDR/flk-1表达的影响  

Influence of Nerve Growth Factor Pretreatment on expression VEGF and KDR/flk-1 in AMI rats

作　　者：李颖[1] 孙新[2] 郭华涛[1] 熊英[2] 翟羽佳[1] 徐雷[1] 

机构地区：[1]北华大学附属医院心内科,吉林吉林市132011 [2]北华大学生命科学中心,吉林吉林市132011

出　　处：《中国实验诊断学》2011年第10期1638-1640,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省自然科学基金项目(20105113)

摘　　要：目的研究神经生长因子(NGF)预处理对急性心肌梗死(AMI)大鼠心肌中VEGF及其受体KDR/flk-1表达的影响。方法雄性成年Wistar大鼠60只,随机分为3组:NGF预处理组和非NGF预处理组和对照组(每组20只)。大鼠NGF预处理使用注射用鼠神经生长因子(0.5μg/kg)尾静脉注射。采用电凝烧灼左冠状动脉前降支制作大鼠急性心肌梗死动物模型。观察各组大鼠心肌损伤的病理改变;以RT-PCR方法检测大鼠心肌组织中血管内皮生长因子(VEGF)及其受体KDR/flk-1的表达情况。结果大鼠心肌坏死面积组间比较差异有统计学意义(F=12.13,P<0.01),NGF预处理组(25.41±9.06)%明显少于非NGF组(40.08±11.38)%,P<0.01。大鼠心肌VEGF、KDR/flk-1mRNA表达组间比较差异有统计学意义(F=18.23,14.10,P均<0.01);NGF预处理组VEGF(45.41±11.54)、KDR/flk-1(47.40±7.83)均高于非NGF组VEGF(32.34±8.10)、KDR/flk-1(31.09±7.88),P均<0.01。大鼠心肌VEGF mRNA表达与其受体KDR/flk-1 mRNA表达呈正相关关系(r=0.691,P<0.01)。结论 NGF预处理可能通过上调VEGF及其受体表达,增加大鼠心肌中新生血管密度,减轻AMI大鼠心肌损伤。Objective To investigate the effects of NGF（Nerve Growth Factor） pretreatment on expression VEGF and KDR/flk-1 of acute myocardial infaction in rats.Methods 60 male adult Wistar rats were randomly classified into three groups： the control（n=20）,non-NGF（n=20） and NGF（n=20） groups.The NGF group was injected NGF（0.5 μg/kg） via caudal vena.Then we performed left anterior descending coronary artery（LAD） ligation by means of electrotome.In this study we observe the morphologic changes of myocardial pathology in each group;To measure the expression of vascular endothelial growth factor（VEGF） and KDR/flk1 we use immunohistology and RT-PCR.Results Infarct areas of rats had statistically significant differences among groups（F=12.13,P0.01）,but the damage in NGF group（25.41±9.06）% was significantly less than non-NGF group（40.08±11.38）%,P0.01.Differences of VEGF,KDR/flk-1 mRNA expression had statistics significance between groups（F=18.23,14.10,all P0.01）.Compared to non-NGF group VEGF（32.34±8.10）、KDR/flk-1（31.09±7.88） were higher in the NGF group VEGF（45.41±11.54）、KDR/flk-1（47.40±7.83）,all P0.01.A positive correlation was showed between VEGF mRNA and KDR/flk-1 mRNA（r=0.691,P0.01）.Conclusion The NGF pretreaement can enhance the new vessels density in cardiac muscle,through the rise of expression of VEGF and KDR/flk-1,and lessen the myocardial damage induced by AMI in rats.

关 键 词：心肌梗死 大鼠 神经生长因子 血管内皮生长因子 KDR/FLK-1 

分 类 号：R542.22[医药卫生—心血管疾病]