糖尿病神经病理性痛大鼠脊髓背角凋亡细胞与凋亡蛋白酶caspase-3的表达变化  

The expressions of apoptotic cells and caspases-3 in rats with diabetic neuropathological pain

作　　者：吴川[1] 王秀丽[1] 王倩[1] 刘彦涛[1] 董蕊[1] 刘朋[1] 

机构地区：[1]河北医科大学第三医院麻醉科,石家庄市050051

出　　处：《河北医药》2011年第22期3365-3368,共4页Hebei Medical Journal

基　　金：国家自然科学基金(编号:30772082);河北省自然科学基金(编号:C2008000931);河北省卫生厅资助课题(编号:07090;20100105)

摘　　要：目的探讨糖尿病神经病理性痛(DNP)大鼠脊髓背角凋亡细胞及与之相联系的凋亡蛋白酶caspase-3的表达变化。方法健康雄性SD大鼠60只,随机分为DNP组和正常对照组,每组30例。DNP组:腹腔注射链脲霉素(STZ)50 mg/kg制备成DNP模型;正常对照组给予等容量0.9%氯化钠溶液。分别给予注射STZ或0.9%氯化钠溶液后3、5、7周时取10只大鼠,采集静脉血样,测定空腹血糖浓度,然后测定机械缩足阈值,取2组大鼠L1-5脊髓组织,采用原位末端标记法(TUNEL)检测2组大鼠脊髓背角神经细胞凋亡的变化,采用免疫组织化学染色法观察凋亡蛋白酶caspase-3的表达变化。结果与正常对照组比较,DNP组血糖升高,机械缩足阈值降低(P<0.05),凋亡细胞与凋亡蛋白酶caspase-3的表达逐渐增加(P<0.05)。结论 DNP大鼠脊髓背角凋亡细胞与凋亡蛋白酶caspase-3的表达上调。Objective To investigate the expressions of apoptotic cells and caspaes-3 in the spinal dorsal horn of rats with diabetic neuropathologieal pain （DNP）. Methods Sixty male SD rats aged 4 weeks, weighing 150 - 170g were randomly divided into two groups, with 30 rats in each group：eontrol group （ group C） and DNP group （group D）. The DNP models of rats were established by single intraperitoneal injeetion of streptozotocin （STZ,50mg/kg） in group D,and the equal volume of normal saline was injected instead of STZ in group C. The fasting blood glucose levels and paw withdraw threshold （PWT） were detected at 3,5,7 weeks after the injection. The 10 rats were taken out in each group at every time point,and the Ll-5 myeloid tissues were taken out,then the expressions of apoptotic cells in spinal dorsal horn of the rats were detected by Tunnel and the expression of caspase-3 was detected by immunohistochemistry. Results As compared with those in group C, the average fasting blood glucose levels in group D were significantly increased, however, the PWT was obviously decreased at 3, 7 weeks after the injection （ P 〈 0.05）, however, the expressions of apoptotic cells and caspases-3 in group D were significandy higher than those in group C at 3,5,7 weeks after injection （ P 〈 0.05）. Conclusion The expressions of apoptotic cells and caspases-3 are up-regulated in the spinal dorsal horn of STZ-induced DNP rats.

关 键 词：糖尿病神经痛 脊髓背角 细胞凋亡 caspase-3 

分 类 号：R587.25[医药卫生—内分泌]