微流控技术在细胞转染中的应用  

Application of microfluidics in cell transfection:a review

作　　者：徐海明[1] 蒋稼欢[1] 

机构地区：[1]重庆大学生物流变科学与技术教育部重点实验室,重庆400044

出　　处：《生物工程学报》2011年第10期1417-1427,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.30870607);重庆市自然科学基金(No.CSTC2008BB5192);科技部国际科技合作重点项目(No.2005DFA00190);国家"111计划"(No.B06023)资助~~

摘　　要：微流控技术是在微米级、纳米级结构中操控纳升至皮升体积流体的技术与科学,具有液体流动可控、消耗试样和试剂极少等优点。近年来,细胞转染技术有逐渐向微型化技术途径发展的趋势,这也给了研究者从微尺度角度审视细胞转染技术过程的新机会。以下介绍了基于微流控技术的细胞转染方法,包括微阵列方式的转染技术、缩微流动空间中的转染、微流控液滴技术应用于细胞转染、微流控注射技术以及微流控电穿孔技术,并阐述了影响转染效率的因素或改善途径。Microfluidics deals with the manipulation of fluidics in the structure with dimensions of micrometers or nanometers.As an emerging field,microfluidics has numerous advantages,such as controllable fluid flow and reduced reagents consumption.Recently,microfluidic has been applied into the area of cell transfection,providing opportunities to investigate cell transfection process on microscale.This review summarizes recent technical development of cell transfection based on microfluidics,including transfeceted microarray,transfecetion established in miniaturization flowing space,microdrops,microinjection and microfluidic electroporation.The factors that affect the transfection efficiency and improvement approaches are also discussed.

关 键 词：微流控 转染 基因 效率 

分 类 号：Q813[生物学—生物工程]