银花苦丁茶饮料对烫伤大鼠肾的保护作用  

Protective effect of honeysuckle immature Chinese holly leaf beverages on the kidney of scalded rats

作　　者：柳洁[1] 肖聪颖[1] 汪冶[1] 陈良富[1] 

机构地区：[1]怀化医学高等专科学校组织学与胚胎学教研室,怀化418000

出　　处：《解剖学杂志》2011年第5期605-608,F0002,共5页Chinese Journal of Anatomy

基　　金：湖南省科技厅社会发展支撑计划项目（2008SK3034）;科技部中医药行业科研专项中药类资助项目（200807020）

摘　　要：目的：观察银花苦丁茶饮料对烫伤大鼠肾微细结构及血清肿瘤坏死因子α（TNFα）与白细胞介素-1（IL-1）浓度的影响，评价其对肾的保护作用。方法：H-E染色法检测肾实质的微细结构变化， SABC免疫组织化学法检测肾实质TNFα表达；ELISA检测血清TNFα与IL-1。结果：与对照组比较，实验组肾实质的炎症与损伤程度轻，范围局限，进展缓慢，血管球淤血而无出血，肾小管上皮内仅见颗粒变性细胞与轻度水样变性细胞，TNFα表达较弱，灰度值与烫伤时间呈负相关，血清TNFα与IL-1浓度在烫伤后缓慢升高，在第4小时达高峰（77.61±15.20， 47.84±2.78）。结论：银花苦丁茶饮料能延缓TNFα与IL-1的释放，对烫伤大鼠肾有一定的保护作用。Objective： To observe the influence of honeysuckle immature Chinese holly leaf beverages on the kidney micro- structure and serum concentration of TNFα and IL-I, and to estimate its protective effect on the kidney. Methods： The microstructural changes of renal parenchyma were detected with HE and the TNFα expression was detected with immunohistochemical SABC. The serum concentration of TNFα and IL-1 was detected with ELISA. Results： Compared with the control group, the inflammation and damage degree of the renal parenchyma was lighter, the range was limited and the condition de- veloped slowly in the experimental group. The glomeruli congested without bleeding. The granular degenerating cells and slight hydropic degenerating cells were seen in the renal tubular epithelia. The expression of TNFa was weaker, and the grey level and time presented negative correlation. The serum concentration of TNFα and IL-1 increased slowly after scalding, which was the highest in the fourth hour （77.61±15.20, 47.84±2.78）. Conclusion： Honeysuckle immature Chinese holly leaf beverages can delay the release of TNFα and IL-1 and have certain protective effect on the kidney of scalded rats, so it can be used as an adjuvant therapy for burns.

关 键 词：金银花 苦丁茶 烫伤 肾 肿瘤坏死因子Α 白细胞介素-1 

分 类 号：R644[医药卫生—外科学]