纤溶活性蛋白检测方法研究进展  

A Review on Analytic Techniques of Fibrinolytic Protein

作　　者：黄明星[1] 叶韵[1] 韩雅莉[1] 

机构地区：[1]广东工业大学轻工化工学院生物工程系,广州510006

出　　处：《中国生物工程杂志》2011年第10期113-120,共8页China Biotechnology

基　　金：国家自然科学基金资助项目(30772739)

摘　　要：迄今为止,对纤溶活性蛋白(fibrinolytic protein)的检测主要有4种方法:纤维平板法(fibrinplate method)、显色底物法(colorimetric assay using chromogenic substrates)、反相纤维蛋白自显影法(reverse fibrin autography)和纤维蛋白酶谱法(fibrin zymography)。纤维平板法可以用来快速判断样品是否具有纤溶活性,同时,纤维平板法和显色底物法也可以用来对纤溶活性蛋白进行半定量分析。反相自显影法和纤维蛋白酶谱法则是两种较新的技术,主要用来对纤溶活性蛋白进行定性分析。详细阐述了这几种技术的发展过程、原理、优缺点和应用范围。PAs are serine proteases of tryptic specificity which can convert the proenzyme,plasminogen,into an active enzyme plasmin.For the assay of PAs activity,four techniques have been established.Fibrin plate method and colorimetric assay using chromogenic substrates are two early methods which can be used for semi-quantitative analysis.Reverse fibrin autography and fibrin zymography are two new qualitative techniques which can be used to detect the molecular weight or isoelectric point of unknown PAs.The developing process and principle of these four techniques were reviewed,and the potential applications,advantages and disadvantages were discussed.

关 键 词：纤溶活性蛋白 纤维平板法 显色底物法 反相自显影法 纤维蛋白酶谱法 

分 类 号：Q819[生物学—生物工程]