心房肽Ⅲ对离体大鼠心脏缺血再灌注损伤的保护作用  

The protective effect of APⅢ on myocardial ischemia-reperfusion injury in rats

作　　者：刘永欣[1] 曹东平[1] 刘奎甲 吴超 彭应心[1] 

机构地区：[1]河北省人民医院心脏二科 [2]河北省博野县医院内科

出　　处：《河北医药》2011年第21期3210-3211,共2页Hebei Medical Journal

摘　　要：目的观察心房肽Ⅲ(APⅢ)对离体大鼠心肌缺血再灌注的保护作用。方法离体心脏停灌30min,造成全心缺血,复灌60min。共取36个心脏随机分为6个组:高、中、低(1×10-7mol/L、3×10-8mol/L、1×10-8mol/L)3个剂量APⅢ组,(缺血再灌注)模型对照组,维拉帕米(10-7mol/L)对照组和正常对照组。测定复灌第20分钟冠脉流出液中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)的活性和灌流结束后心肌组织SOD活性和MDA含量。结果灌流液中,3种剂量的APⅢ组和维拉帕米组,SOD活性均显著高于模型对照组(P<0.05),LDH活性均低于模型对照组,但无统计学意义(P>0.05)。心肌组织中,各剂量APⅢ组和维拉帕米组的SOD活性均高于模型对照组(P<0.01);各剂量APⅢ组和维拉帕米组MDA的含量均低于模型对照组,高、中剂量APⅢ组(P<0.05),而低剂量APⅢ组和维拉帕米组差异无统计学意义(P>0.05)。结论 APⅢ可以减少心脏I/R中氧自由基的生成,保护心肌,作用优于维拉帕米,并有剂量依赖性。Objective To observe the protective effect of atrial natriuretic peptide （ANP 19 ） on myocardial ischemia-reperfusion （I/R） injury in rats. Methods In this experiment, the isolated rat hearts were submitted to 30-minute non-perfusion which resulted in global ischemia, then 60-minute reperfusion. The 36 hearts of rats were randomly divided into 6 groups： high, middle, low-dose （1 ×10^-7mol/L,3 ×10^-8mol/L and 1×10^-8 mol/L） AP 19 groups, verapamil（ 10-7 mol/L）group and model control group. The activities of LDH and SOD in coronary effluence after 20-minute reperfusion, and the activity of SOD and content of MDA in myocardial tissue after reperfusion were detected respectively. Results The activities of SOD in coronary effluence in three different dose APⅢ groups and verapamil group were significantly higher than those in model control group （ P 〈 0.05）, however,the activities of LDH in the fourth groups mentioned above were lower than those in model control group, but there was no significant difference among them （ P 〉 0.05 ）. In myocardial tissue, the activities of SOD in three different dose AP 19 groups and verapamil group were significantly higher than those in model control group （P 〈 0.05 ）, however,the contents of MDA in the four groups were significantly lower than those in model control group,there were significant differences between high-dose, middle-dose AP 19 groups and model control group （P 〈 0.05）, but there was no significant difference between low-dose AP 19 group and verapamil group （ P 〉 0.05）. Conclusion AP 19 can reduce the formation of oxygen radicals due to I/R in heart, and can protect myocardium. Its effect is superior to that of verapamil and is dose-independent.

关 键 词：心房肽Ⅲ 心肌缺血再灌注 氧自由基 

分 类 号：R542.2[医药卫生—心血管疾病]