基于微电极阵列的嗅球细胞网络传感器的研究  

Study on Olfactory Bulb Cell Network Biosensor Based on Microelectrode Array

作　　者：陈庆梅[1,2] 肖丽丹[1] 李蓉[1] 张威[1] 刘清君[1] 王平[1] 

机构地区：[1]浙江大学生物传感器国家专业实验室生物医学工程教育部重点实验室生物医学工程系,杭州310027 [2]江西中医学院医疗电子学科组,南昌330006

出　　处：《中国生物医学工程学报》2011年第5期745-749,共5页Chinese Journal of Biomedical Engineering

基　　金：国家杰出青年科学基金(60725102);国家自然科学基金(30970765);江西省卫生厅科技计划(20092049)

摘　　要：嗅球(OB)是嗅觉系统的第一中转站,在嗅觉信息的识别和处理中具有重要的作用。嗅球中具有多种类型的神经元,分别具有不同的生理特点和功能。本研究利用细胞培养技术,将嗅球神经元与微电极阵列(MEA)芯片耦合,构建一种细胞网络传感器,用于对多点的嗅球神经元电活动进行同步观察与分析。结果显示,MEA上培养的嗅球细胞生长良好,能够检测多个通道的嗅球神经元的自发电位以及谷氨酸作用下的诱发响应。研究表明,该嗅球细胞网络传感器能够实现信号的多通道同步检测及有效分辨神经元的自发信号和诱发响应,并且能够很好地捕捉不同通道神经元响应的特点。该研究对于进一步分析嗅觉信息在嗅球内的传导和编码具有重要的意义。Olfactory bulb（OB） is the first relay site of the olfactory system,which is important for identifying and processing the olfactory information.There are multi-type neurons in OB,respectively with different physiological characteristics and functions.In the present study,OB neurons were coupled onto MEA chip by cell culture technique to develop a cell network-based biosensor,which was used to synchronously observe and analyze the electrical activities of OB neurons with multi-sites.Experiment results showed that OB cells grew well on MEA,and multi-channel spontaneous signals can be measured.In addition,this biosensor can detect the induced spike potentials and oscillation signals by the OB neurons under the action of glutamic acid（Glu）.All results suggest that OB cell network-based biosensor can realize multi-channel synchronous measurement and effectively differentiate the spontaneous signals from the induced responses,and can also catch the characteristics of neuronal responses of different channels commendably.This research is significant for further investigating the conduction and coding functions of olfactory information in the OB.

关 键 词：细胞网络传感器 嗅球 微电极阵列 

分 类 号：R318.5[医药卫生—生物医学工程] TP212.3[医药卫生—基础医学]