人卵巢颗粒细胞的体外培养方法探讨  

Culture method of human ovarian granulosa cells in vitro

作　　者：刘玉霞[1] 董静霞[1] 金玉洁[1] 申春艳[1] 徐健[1] 

机构地区：[1]北京大学基础医学院人体解剖与组织胚胎学系,北京100191

出　　处：《中国组织化学与细胞化学杂志》2011年第5期436-439,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金面上项目(30772344)

摘　　要：目的建立人卵巢颗粒细胞分离纯化、体外培养的有效方法。方法收集体外受精—胚胎移植(IVF-ET)穿卵时的卵泡液,用胰蛋白酶消化法及密度梯度离心法分离纯化颗粒细胞并用不同培养基进行培养。结果用体积分数为50%的Percoll细胞分离液分离,DMEM/F12或McCoy’5a液体培养基进行培养,细胞纯度高,存活率高,后续生长良好。结论建立了人卵巢颗粒细胞体外培养的稳定模型,为颗粒细胞的体外研究奠定良好的基础。Objective To establish effective methods of purification and culture in vitro of human ovarian granulosa cells. Methods Human ovarian granulosa cells were obtained in conjunction with oocyte aspiration from women undergoing hormone treatment for in vitro fertilization.Cells were treated with trypsin and density gradient centrifugation,and then cultured with different media. Results Centrifuged by 50%（v/v） Percoll PLUS,and cultured with DMEM/F12 or McCoy＇5a liquid medium,cells were of great purity,high survival rate,and good subsequent growth. Conclusion We have established a stable model of culturing human ovarian granulosa cells in vitro,and laid a good foundation for research of granulosa cells in vitro.

关 键 词：人 卵巢颗粒细胞 细胞培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]