Induction of Endothelial Cell Apoptosis by Anti-alpha-enolase Antibody  

作　　者：Hong-bo Yang Wen-jie Zheng Xuan Zhang Fu-lin Tang 

机构地区：[1]Department of Endocrinology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences ＆ Peking Union Medical College,Beijing 100730,China [2]Department of Rheumatology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences ＆ Peking Union Medical College,Beijing 100730,China

出　　处：《Chinese Medical Sciences Journal》2011年第3期152-157,共6页中国医学科学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China (30400411)

摘　　要：Objective To assess the prevalence of anti-alpha-enolase antibody in systemic autoimmune diseases in Chinese patients and its role in endothelial cell apoptosis.Methods The reactivity of anti-alpha-enolase antibody in a variety of autoimmune disorders in Chinese patients was evaluated by dot blot assay.Endothelial cell apoptosis was investigated by in vitro incubation of endothelial cells with IgG purified from anti-alpha-enolase antibody-positive sera,with or without pre-incubation with recombinant alpha-enolase.Results Anti-alpha-enolase antibody was prevalent in different systemic autoimmune diseases with relatively high reactivity in Chinese patients.In vitro incubation of endothelial cells with IgG containing anti-alpha-enolase antibody induced apoptosis in a time-and dose-dependent manner.Apoptosis was partly inhibited by pre-incubation of the endothelial cells with recombinant alpha-enolase.Conclusions Our data suggest that alpha-enolase is a common auto-antigen recognized by anti-endothelial cell antibodies in connective tissue disease.Interaction between alpha-enolase and its autoantibody plays a role in endothelial cell apoptosis.Changes other than cell killing may contribute to the pathogenesis of endothelial damage and microvascular lesions.

关 键 词：anti-alpha-enolase antibody apoptosis endothelial cell 

分 类 号：S858.285.3[农业科学—临床兽医学] Q55[农业科学—兽医学]