Mina基因单核苷酸多态性与儿童变应性哮喘关联研究  

Associations of Mina gene polymorphisms with children atopic asthma in chongqing Han population

作　　者：陈芸[1] 杨锡强[1] 黄英[1] 刘恩梅[1] 王莉佳[1] 

机构地区：[1]重庆医科大学附属儿童医院免疫研究室,重庆400014

出　　处：《重庆医学》2011年第31期3121-3123,3126,共4页Chongqing medicine

基　　金：国家"十一五"科技支撑计划项目(2007BAI24B05)

摘　　要：目的研究Mina基因单核苷酸多态性(SNPs)与重庆地区汉族儿童变应性哮喘的相关性。方法采用病例对照研究。基质辅助激光解吸电离飞行间质谱技术(MALDI-TOF)进行Mina位点基因分型,以观察各位点不同基因型和等位基因频率在哮喘组和对照组中的分布差异。显性和隐性遗传模型用来比较哮喘组和正常组rs4857304位点基因型之间的差异。结果共纳入支气管哮喘患儿202例,对照组191例。Mina的rs4857304位点在哮喘组和对照组等位基因频率比较,差异有统计学意义(P=0.019 9),哮喘组rs4857304位点等位基因T频率高于对照组。通过隐性遗传模型分析发现,rs4857304位点主要等位基因T纯合型,即TT型和GG+GT型相比,在两组比较差异有统计学意义(P=0.028 2,OR=1.568,95%CI=1.048～2.346)。结论Mina基因rs4857304位点与儿童变应性哮喘相关。Objective To study the associations of the single-nucleotide polymorphisms polymorphisms（SNPs）of Mina gene rs832081,rs832078,rs4857304,rs9879532,rs17374916 rs1532206 with children asthma in Chongqing Han people.Methods A case-control study was applied to observe the distributions of SNPs of Mina gene in the asthmatic patients and controls.202 cases of asthmatic children and 191 controls were included.SNP genotyping was performed by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method（MALDI-TOF）.The recessive and dominant genetic model analysis was used to study the differences of rs4857304 genotypes.Results Among the six SNPs,only rs4857304 of Mina gene of wheezing patients presented with a significantly higher portion of T allele frequencies（P=0.0199）.By the recessive genetic model analysis found that the proportion of the main allele homozygous TT of rs4857304 was higher than the proportion of GT＋TT type（P=0.0282,OR=1.568,95%CI=1.048-2.346）.Conclusion Mina gene rs4857304 has high relationship with children atopic asthma.

关 键 词：儿童 哮喘 基因型 Mina基因 

分 类 号：R725.6[医药卫生—儿科]