石榴花二氢黄酮醇还原酶(DFR)基因RT-PCR扩增体系的建立与优化  

Establishment and Optimization of RT-PCR System for DFR in Pomegranate Flower

作　　者：招雪晴[1] 苑兆和[1] 陶吉寒[1] 尹燕雷[1] 冯立娟[1] 

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《山东林业科技》2011年第5期20-22,共3页Journal of Shandong Forestry Science and Technology

基　　金：山东省自然基金项目(ZR2009DM010);国家外专局项目[鲁外专(2010)18号];山东省农业科技成果转化项目[鲁科农字(2010)79号]

摘　　要：分别以红花和粉花石榴‘泰山红’、‘重瓣粉花’盛花期花瓣为材料,提取RNA后进行反转录,对DFR基因的PCR扩增体系进行了优化,建立了高效、特异的石榴花DFR基因的RT-PCR扩增体系,为克隆基因全长奠定了基础。Total RNA was extracted from red and pink pomegranate flowers and reverse transcription were carried out re spectively. The PCR system for amplification of DFR gene was optimized. An effective and specific amplification system was established. This work provided the foundation for gene cloning.

关 键 词：石榴 DFR RT—PCR 

分 类 号：S665.4[农业科学—果树学]