HPLC-ESI-MS^2法测定制药废水中青霉素G残留  

Simultaneous Determination of PEN G in Pharmaceutical Wastewater by HPLC-ESI-MS^2

作　　者：康全影[1,2] 朱晓磊[1,2] 张媛[1,3] 

机构地区：[1]河北省环境科学研究院,河北石家庄050037 [2]河北省水环境科学实验室,河北石家庄050037 [3]武汉大学资源与环境科学学院环境工程系,湖北武汉430079

出　　处：《广州化工》2011年第20期85-87,共3页GuangZhou Chemical Industry

基　　金：河北省科技支撑计划项目(No:76711D)

摘　　要：建立了一种测定制药废水中青霉素G残留的高效液相色谱-电喷雾质谱方法。样品经Octadecyl C18固相萃取柱净化后,以乙腈-水-乙酸(体积比20∶80∶0.005)体系溶液作为流动相,电喷雾质谱检测器进行定性和定量分析。该方法检出限为5μg/L,相对标准偏差(RSD%)为6.2%,回收率是64.9%～76.3%。实验结果表明,该方法具有准确、快速的特点,适合制药废水中青霉素G残留的测定。A method was developed for the simultaneous determination of PEN G in pharmaceutical wastewater by high performance liquid chromatography-electrospray tandem mass spectrometry（HPLC-ESI-MS2）.The sample was purified by Octadecyl C18 solid-phase extraction column.The high performance liquid chromatograph（HPLC） separation was performed with acetonitrile-water-acetic acid（volume ratio 20：80：0.005） as mobile phase.The identification and quantification were done with mass spectrometry.The limit of detection was 5 μg/L.The recovery ranged from 64.9% to 76.3% with relative standard deviation 6.2%.The experimental results showed that this method was accurate and rapid,which can be used for the residue analysis of PEN G in pharmaceutical wastewater.

关 键 词：高效液相色谱 电喷雾串联质谱 青霉素G 制药废水 

分 类 号：O657.72[理学—分析化学]