糖脉康颗粒清除DPPH自由基的作用  

DPPH Radical Scavenging Effect of Compound Tangmaikang

作　　者：赵启鹏[1,2] 兰莎[3] 张艺[3] 

机构地区：[1]宁夏回药现代化工程技术研究中心,银川750004 [2]宁夏医科大学药学院,银川750004 [3]成都中医药大学民族医药学院,成都611137

出　　处：《中国实验方剂学杂志》2011年第21期41-45,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划项目(2007BAI40B03)

摘　　要：目的:建立体外清除DPPH方法,评价糖脉康颗粒体外抗氧化作用。方法:对清除DPPH方法进行反应时间,反应温度,DPPH的浓度与吸光度的关系,糖脉康的提取溶剂,提取方法等考察,对14批糖脉康及含药血清进行体外清除DPPH实验。结果:糖脉康以60%甲醇为溶剂,超声提取30 min,与0.099 8 g.L-1的DPPH于60℃反应50 min;14批糖脉康颗粒对DPPH的平均IC50=0.48 mg(生药量);含药血清对DPPH清除率与空白血清比有所下降。结论:糖脉康颗粒具有体外抗氧化作用。Objective：To establish a method of scavenging DPPH radical,and then evaluate DPPH radical scavenging effect of compound Tangmaikang.Method： Investigated the reaction time,temperature,the concentration of DPPH and the extraction solvent and method,the study on the DPPH radical scavenging effect of 14 batches Tangmaikang and drug-containing serum was carried out.Result： Tangmaikang was extracted with 60% methanol by ultrasonic for 30 min.The optimum reaction conditions were obtained as follows：DPPH concentration 0.099 8 g·L-1,reaction temperature 60 ℃,reaction time 50 min.The IC50 of 14 batches of Tangmaikang was 0.48 mg.The DPPH clearance of drug-containing serum was lower than blank serum.Conclusion： Tangmaikang has antioxidant activity in vitro.

关 键 词：糖脉康颗粒 DPPH法 抗氧化 

分 类 号：R284.1[医药卫生—中药学]