微波协助提取在中药饮片含量测定中的应用(1)——微波法与药典法测定黄芩中黄芩苷含量比较研究  

Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs(1) Comparison of the Determination of Baicalin from Scutellaria baicalensis between Microwave-assisted Extraction and Chinese Pharmacopeia Method

作　　者：方婧[1] 付梅红[1] 杨洪军[1] 张贝贝[1] 王祝举[1] 杨岚[1] 张东[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第21期46-48,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划(2006BAI09B07-02)

摘　　要：目的:建立微波协助提取法提取黄芩中有效成分黄芩苷,比较微波协助提取法与药典法提取黄芩苷优势。方法:采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水-磷酸(47∶53∶0.2),检测波长280nm,柱温30℃,流速1 mL.min-1。结果:微波提取时间15 min,提取温度120℃,提取溶剂30%乙醇。黄芩苷在0.059～0.59μg呈良好线性关系,r=0.999 9。平均回收率99.36%(n=6)。结论:微波提取法较药典法提取黄芩苷更简便快速、结果准确,为一种符合环保绿色理念的含量测定方法,可用于检测黄芩饮片中有效成分黄芩苷含量。Objective：To establish a microwave-assisted extraction（MAE）method for the extraction of the baicalin from Scutellaria baicalensis.Comparison of the determination of baicalin from S.baicalensis between MAE and Chinese pharmacopeia method.Method：Dikma Diamonsil C18 column（4.6 mm×150 mm,5 μm）was used.The mobile phase of methanol-water-phosphoric acid（47∶53∶0.2）.The temperature of column was 30 ℃.The flow rate was 1.0 mL·min-1 and the detection wavelength was at 280 nm.Result：Microwave extractive time was 15 min,the temperature was 120 ℃,the extraction solvent was 60%ethanol.The calibration curve was in good linearity with in the range from 0.059 to 0.59 μg（r=0.999 9）.The average recovery was 99.36%（n=6）.Conclusion：The method is simple,rapid,as well as precise and reliable,and can be used for the determination of baicalin in S.baicalensis.

关 键 词：微波提取 黄芩 黄芩苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]