三七提取液中三七皂苷R_1、人参皂苷Rg_1和人参皂苷Rb_1的透皮规律研究  

Study on Percutaneous Absorption of Panax notoginseng R_1, Ginsenoside Rg_1 and Ginsenoside Rb_1 through Guinea Pig's Complete Skin in vitro

作　　者：何百寅[1] 谢友良[1,2] 刘常青[1] 叶雪兰[1] 吴安国[1] 陈建南[1,2] 

机构地区：[1]广州中医药大学新药研究开发中心,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中国实验方剂学杂志》2011年第21期130-134,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：东莞市科技计划重点项目(201010810203)

摘　　要：目的:研究三七提取液中三七皂苷R1,人参皂苷Rg1和人参皂苷Rb1等成分对完整豚鼠腹皮的透过性。方法:采用Franz扩散池进行体外透皮实验,以完整豚鼠腹皮为渗透屏障,HPLC法测定三七皂苷R1,人参皂苷Rg1和人参皂苷Rb1的透过量。结果:未加透皮促进剂时,三七提取液中三七皂苷R1,人参皂苷Rg1和人参皂苷Rb1等均不能透过完整豚鼠皮。在氮酮的促进下,三七提取液中的上述成分均可透过完整豚鼠腹皮,其透皮能力:人参皂苷Rg1>三七皂苷R1>人参皂苷Rb1;透皮促进剂氮酮加入量为2%时,上述成分的透皮作用较好。结论:三七提取液中三七皂苷R1,人参皂苷Rg1和人参皂苷Rb1在2%氮酮促进下渗透效果较佳。Objective：To studying transdermal absorption patterns of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 from extraction of Panax notoginseng in vitro.Method： Using Franz diffusion cell for in vitro transdermal experiments,complete guinea pigs＇ abdomen skin was used as transdermal barrier;HPLC was applied to determent the amount of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 which penetrated the test skin.Result： When no penetration enhancers like azone exsited,notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 couldnot pass through test skin at all.When 2% azone was add in the Panax notoginseng extraction,notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 could penetrate the test skin effectively.The sequence of its penetration capabilities was as follows： ginsenoside Rg1 notoginsenoside R1 ginsenoside Rb1.Conclusion： With the assisting of 2% Azone,notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 can effectively penetrate complete guinea pigs＇ skin.

关 键 词：三七提取液 三七皂苷R1 人参皂苷Rg1 人参皂苷RB1 氮酮 透皮吸收 

分 类 号：R285.5[医药卫生—中药学]