静安口服液对小儿多发性抽动症模型大鼠脑内多巴胺转运体分布的影响  

Effect of Jing'an Oral Liquid on Distribution of Dopamine Transporter in Brain of Rat Model with Tourette Syndrome

作　　者：隆红艳[1] 张骠[2] 

机构地区：[1]南京中医药大学,南京210046 [2]南京市中医院儿科,南京210001

出　　处：《中国实验方剂学杂志》2011年第21期181-183,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：高等学校博士学科点专项科研基金项目(20070315009);南京中医药大学中医儿科学学科开放课题(EZK2009018)

摘　　要：目的:探讨小儿多发性抽动症(Tourette综合征,TS)发病与突触前多巴胺神经纤维支配的关系,观察静安口服液对小儿多发性抽动症模型大鼠脑内多巴胺转运体(DAT)分布的影响。方法:ip DOI[选择性5-HT(2A/2C)受体激动剂,1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane]1 mg.kg-1,连续21 d,诱导大鼠抽动模型,以出现头部抽动、耸肩及口腔运动、理毛、舔食前爪等刻板动作作为该模型诱导成功的观察指标。分为空白对照组、模型组、静安口服液组(11 g.kg-1)、硫必利组(0.025g.kg-1)4组,ig给药21 d。各组以99 Tc-TRODAT-1为配体,采用γ-放射免疫计数器测定大鼠脑内DAT分布。结果:模型组纹状体、大脑皮层、海马3个部位放射性摄取比值(CMP/GB)均比对照组明显增高(P<0.05),静安口服液组3个部位放射性摄取比值(CMP/GB)均比模型组明显减少(P<0.05),具有统计学差异。结论:Tourette综合征模型大鼠脑内多巴胺转运体分布较正常大鼠显著增多,致使多巴胺功能亢进,导致Tourette综合征发生;静安口服液可能通过减少脑内DAT分布,从而降低突触前多巴胺神经元的过度支配而发挥治疗作用。Objective：To Explore the relationship between pathogenesis of Tourette syndrome（TS） and dominant with presynaptic dopamine nerve fibers.At the same time to observe the effect of Jing＇an oral liquid on distribution of dopamine transporter of TS model rats.Method： The model of TS was established by peritoneal injection of 1-（2,5-dimethoxy-4-iodophenyl）-2-aminopropane（DOI） 1 mg·g-1 for 21 days.SD rats were randomly divided into 4 groups,the control group,the model group,Jing＇an oral liquid group（11 g·kg-1） and tiapride group（0.025 g·kg-1）,and drugs were orally administered for 21 days.In each group with 99 Tc-TRODAT-1 as a ligand,the distribution of dopamine transporter in rat brain was measured by γ-radiation immunity Counter.Result： In model group,three sites＇（striatum,cerebral cortex and hippocampus） radioactive uptake ratio（CMP / GB） was significantly higher than that in control group（P0.05）.In Jing＇an oral liquid group three sites＇ radioactive uptake ratio（CMP/GB） was lower than that in model group（P0.05） with a statistically significant difference.Conclusion： Compared with normal group,the distribution of dopamine transporter（DAT） in model group brain significantly is increased,which may cause the dopamine hyperfunction and lead to TS,.Jing＇an oral liquid may be play a therapeutic role by reducing the distribution of DAT,thereby reducing the presynaptic dopamine neurons over-dominance.

关 键 词：静安口服液 多发性抽动症 多巴胺转运体 大鼠 

分 类 号：R285.5[医药卫生—中药学]