生姜水提物对全脑缺血再灌注大鼠脑组织氨基酸递质的影响  

Effect of Aqueous Extract from Zingiberis Rhizoma Recens on Excitatory Amino Acid in Brain Tissue of Cerebral Ischemia Reperfusion in Rats in vivo

作　　者：王军[1] 黄启福[2] 刘惠霞[1] 张薇[1] 

机构地区：[1]河南省中医药研究院,郑州450004 [2]北京中医药大学基础医学院,北京100029

出　　处：《中国实验方剂学杂志》2011年第21期184-187,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省属科研单位社会公益项目预研专项资金(0641130503)

摘　　要：目的:研究生姜水提物对全脑缺血再灌注大鼠脑组织氨基酸递质的影响。方法:SD雄性大鼠随机分为假手术组、模型对照组、尼莫地平组(30 mg.kg-1)、生姜高、中、低剂量组(200,100,50 mg.kg-1)。Pulsinelli’s四动脉阻断法造成全脑缺血再灌注损伤模型(CIR),分别于手术前1 d,术前1 h和再灌注前30 min ig给药,共3次。高效液相色谱法测定脑组织谷氨酸(Glu)、天冬氨酸(Asp)和甘氨酸(Gly)含量,原子吸收分光光度计测定脑组织Ca2+含量,干湿重法测定脑组织含水量,化学比色法测定脑组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与假手术组比较,CIR模型组大鼠脑组织Glu,Ca2+,MDA含量和含水量明显升高(P<0.05或P<0.01),SOD活性明显降低(P<0.05);生姜水提物能显著降低脑组织Glu,Ca2+含量和含水量(P<0.05或P<0.01),显著升高SOD活性和SOD/MDA比值(P<0.05)。结论:生姜水提物防治脑缺血再灌注损伤的机制与降低兴奋性氨基酸(EAA)兴奋性毒性、阻滞Ca2+超载和提高抗氧化活性有关。Objective：To investigate the effects of aqueous extract from Zingiberis Rhizoma Recens on excitatory amino acid（EAA） in brain tissue after cerebral ischemia reperfusion（CIR） in rats.Method： Male SD rats were randomly assigned to 6 groups as following：sham-operation group,model control group,nimodipine group and high,middle and low dosage groups of aqueous extract from Zingiberis Rhizoma Recens.CIR model in rats was reproduced by Pulsinelli＇s ＂Four Vessel Occlusion＂ method.The contents of glumatate（Glu）,asparate（Asp）,and glycine（Gly） in brain tissue were analyzed by high performance liquid chromatography with fluorescent detection.Atomic spectrophotometry was used to measure Ca2＋ content in brain tissue.The changes of brain water content were measured by the wet and dry weight methods.Spectrophotometric assay was used to measure the activity of superoxide dismutase（SOD） and the contents of malondialdehyde（MDA） in brain tissue.Result： The contents of Glu,Ca2＋,water,and MDA in brain tissue were increased,the activitiyof SOD was declined significantly in CIR model group,compared with the sham operation group.Aqueous extract from Zingiberis Rhizoma Recens could decrease the contents of Glu,Ca2＋ and water in brain tissue,increase the activities of SOD,but there was no significant differencein the content of Gly,compared with model control group.Conclusion： The action of aqueous extract from Zingiberis Rhizoma Recens on CIR may be involve in declinning EAA neurotoxicity,decreasing intracellular Ca2＋ overload and increasing antioxidant activity.

关 键 词：生姜水提物 脑缺血再灌注 兴奋性氨基酸 

分 类 号：R285.5[医药卫生—中药学]