HIV-1Vpr体外诱导肿瘤细胞凋亡与细胞周期阻滞的研究  

Cell cycle arrest and cell apoptosis induced by HIV-1 Vpr in cancer cells in vitro

作　　者：佟加贝[1] 韩金祥[1] 鲁艳芹[1] 

机构地区：[1]山东省医药生物技术研究中心,济南250062

出　　处：《国际肿瘤学杂志》2011年第10期794-797,共4页Journal of International Oncology

基　　金：国家高技术研究发展计划项目（2007AA021004）

摘　　要：目的研究HIV-1Vpr诱导Hela、Lovo、HepG2细胞凋亡和G2期阻滞效果。方法构建含有HIV-1Vpr基因的pcDNA4-Vpr重组载体，体外转染Hela、Lovo、HepG2细胞，同时设pcDNA4-EGFP质粒转染对照组、FUGENE组和空白对照组。转染后24、48、72h，通过甲臌化合物法检测细胞增殖，流式细胞仪检测细胞周期分布，并使用ANNEXINv／Pl双染法检测细胞凋亡。结果与对照组相比，转染后24、48、72h，Hela、Lovo、HepG2三种细胞的增殖抑制率、G，期阻滞率及细胞凋亡率均呈上升趋势（P〈0．05）。Hela、Lovo、HepG2在转染后72h的增殖抑制率分别为29．67％、27．35％、31．67％；G2期细胞周期阻滞率分别为24．9％、18．8％、32．1％；凋亡率分别为15．46％、7．7％、41．5％。结论HIVVpr体外能诱导Hela、Lovo、HepG2肿瘤细胞的G2期阻滞和细胞凋亡。Objective To investigate the G2 cell cycle arrest and apoptosis induced by HIV-1 Vpr gene on Hela, Lovo and HepG2 cancer cells. Methods Recombinant vector pcDNA4-Vpr was constructed by DNA recombination technology and was then transfected into Hela, Lovo and HepG2 ceils. Meanwhile, pcDNA4- EGFP plasmid group, FUGENE group and blank group were also set up as control. Ratio of cytostasis was evaluated by MTS assay 24 h, 48 h and 72 h later, cell cycle arrest examined by flow cytometry and apoptosis detected by staining with ANNEXIN V and PI double dyes. Results Compared to the control group, the value of inhibition ratio, G2 arrest and apoptosis of Hela, Lovo and HepG2 cells increased obviously 24 h, 48 h and 72 h after the transfection （ P 〈 0.05 ）. 72 h after the transfection, the inhibition ratio of Hela, Lovo and HepG2 was 29.67%, 27.35% and 31.67% respectively. Percentage of G2 phase cells was 24.9%, 18.8% and 32.1% respectively. Apoptosis percentage of Hela, Lovo and HepG2 cells was 15.46% , 7.7% and 41.5% correspondingly. Conclusion HIV-1 Vpr gene can induce cell cycle G2 arrest and apoptosis of Hela, Lovo and HepG2 cell lines in vitro.

关 键 词：HIV—1 细胞周期 细胞凋亡 

分 类 号：R285[医药卫生—中药学]