HPLC法检测家兔血浆中地克珠利含量  

HPLC Method for the Determination of Diclazuril in Rabbit Plasma

作　　者：杨丽萍[1] 高淑霞[1] 张秀玲[1] 孙海涛[1] 姜文学[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室,济南250100

出　　处：《中国兽药杂志》2011年第10期25-27,共3页Chinese Journal of Veterinary Drug

基　　金：国家兔产业技术体系(项目编号:CARS-44-B-5)

摘　　要：建立了快速、稳定的家兔血浆中地克珠利提取方法,为进一步研究家兔组织中地克珠利的残留量提供基础。采用HPLC法以妥曲珠利为内标,N,N-2甲基甲酰胺及乙腈联合沉淀法提取家兔血浆中地克珠利,色谱柱为Hypersil ODS(2.5μm,250 mm×4.6 mm),流动相为乙腈∶水∶三乙胺∶冰乙酸=55∶45∶0.2∶0.12(V/V),流速为1.0 mL/min,紫外检测波长为240 nm。在0.156～20.0μg/mL浓度范围内,地克珠利的峰面积与其浓度呈现良好的线性关系(r2=0.999 2);回收率为98.9%～102.4%;方法精密度RSD最大值为6.8%;最低检测限为0.078μg/mL。DMF及乙腈联合沉淀法适用于家兔血浆中的地克珠利的提取。To establish a rapid and stable tissue extraction method for the determination of diclazuril residues in rabbit plasma,diclazuril and toltrazuril（internal standard）were added in blank rabbit plasma,extracted by N,N-dimethylformamide（DMF）/acetonitrile precipitation method and determined by HPLC（Hypersil ODS-C18）.The mobile phase consisted of a mixture of tirethylamine/water/triethylamine/aceticacid（55∶ 45∶ 0.2∶ 0.12） at the flow rate was 1.0 mL/min.The ultraviolet detection wavelength was set at 240 nm.The method was proven to be linear over a plasma diclazuril concentration range of 0.156 to 20.0 μg/mL with a mean correlation coefficient of 0.999 2.The recovery of diclazuril from rabbit plasma was found to be 98.9%~102.4%.The max RSD of method precision was found to be 6.8%.The detection limit was 0.078 μg/mL.DMF/acetonitrile precipitation method is suitable for the extraction of diclazuril in rabbit plasma.

关 键 词：家兔 血浆 地克珠利 HPLC 

分 类 号：S859.84[农业科学—临床兽医学]